| Evolution of nacre: biochemistry and proteomics of the shell organic matrix of the cephalopod Nautilus macromphalus. | |
| | |
MedLine Citation:
|
PMID: 19472248 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
In mollusks, one of the most widely studied shell textures is nacre, the lustrous aragonitic layer that constitutes the internal components of the shells of several bivalves, a few gastropods,and one cephalopod: the nautilus. Nacre contains a minor organic fraction, which displays a wide range of functions in relation to the biomineralization process. Here, we have biochemically characterized the nacre matrix of the cephalopod Nautilus macromphalus. The acid-soluble matrix contains a mixture of polydisperse and discrete proteins and glycoproteins, which interact with the formation of calcite crystals. In addition, a few bind calcium ions. Furthermore, we have used a proteomic approach,which was applied to the acetic acid-soluble and -insoluble shell matrices, as well as to spots obtained after 2D gel electrophoresis. Our data demonstrate that the insoluble and soluble matrices, although different in their bulk monosaccharide and amino acid compositions, contain numerous shared peptides. Strikingly, most of the obtained partial sequences are entirely new. A few only partly match with bivalvian nacre proteins.Our findings have implications for knowledge of the long-term evolution of molluskan nacre matrices. |
| | |
Authors:
|
Benjamin Marie; Frédéric Marin; Arul Marie; Laurent Bédouet; Lionel Dubost; Gérard Alcaraz; Christian Milet; Gilles Luquet |
Related Documents
:
|
6319628 - Darpp-32, a dopamine- and adenosine 3':5'-monophosphate-regulated phosphoprotein enrich... 2430278 - Purification and partial characterization of prostate-derived growth factor. 21815408 - Terpenoid metabolites from spongia spp. and their effects on nucleic acid biosynthesis ... 10931178 - Purification, characterization and amino-acid sequence analysis of a thermostable, low ... 8134918 - Induction of cytochrome p4504a by the peroxisome proliferator perfluoro-n-octanoic acid. 16917808 - Antioxidants in thermally treated buckwheat groats. |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: Chembiochem : a European journal of chemical biology Volume: 10 ISSN: 1439-7633 ISO Abbreviation: Chembiochem Publication Date: 2009 Jun |
Date Detail:
|
Created Date: 2009-06-17 Completed Date: 2009-09-30 Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 100937360 Medline TA: Chembiochem Country: Germany |
Other Details:
|
Languages: eng Pagination: 1495-506 Citation Subset: IM |
Affiliation:
|
UMR CNRS 5561 Biogéosciences, Université de Bourgogne, 6 Bd Gabriel, 21000 Dijon, France. benjamin.marie@u-bourgogne.fr |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Amino Acid Sequence Animals Calcium Carbonate / chemistry Chromatography, High Pressure Liquid Evolution* Nautilus / chemistry* Proteins / analysis*, chemistry, isolation & purification Proteome / analysis*, chemistry, isolation & purification Proteomics / methods* Sequence Alignment Solubility Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization |
| Chemical | |
Reg. No./Substance:
|
0/Proteins; 0/Proteome; 471-34-1/Calcium Carbonate |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: 1,n-glycols as dialdehyde equivalents in iridium-catalyzed enantioselective carbonyl allylation and ...
Next Document: Identification of OxyE as an ancillary oxygenase during tetracycline biosynthesis.