Document Detail

Evolution in the laboratory: the genome of Halobacterium salinarum strain R1 compared to that of strain NRC-1.
MedLine Citation:
PMID:  18313895     Owner:  NLM     Status:  MEDLINE    
We report the sequence of the Halobacterium salinarum strain R1 chromosome and its four megaplasmids. Our set of protein-coding genes is supported by extensive proteomic and sequence homology data. The structures of the plasmids, which show three large-scale duplications (adding up to 100 kb), were unequivocally confirmed by cosmid analysis. The chromosome of strain R1 is completely colinear and virtually identical to that of strain NRC-1. Correlation of the plasmid sequences revealed 210 kb of sequence that occurs only in strain R1. The remaining 350 kb shows virtual sequence identity in the two strains. Nevertheless, the number and overall structure of the plasmids are largely incompatible. Also, 20% of the protein sequences differ despite the near identity at the DNA sequence level. Finally, we report genome-wide mobility data for insertion sequences from which we conclude that strains R1 and NRC-1 originate from the same natural isolate. This exemplifies evolution in the laboratory.
F Pfeiffer; S C Schuster; A Broicher; M Falb; P Palm; K Rodewald; A Ruepp; J Soppa; J Tittor; D Oesterhelt
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Publication Detail:
Type:  Journal Article     Date:  2008-03-03
Journal Detail:
Title:  Genomics     Volume:  91     ISSN:  1089-8646     ISO Abbreviation:  Genomics     Publication Date:  2008 Apr 
Date Detail:
Created Date:  2008-03-31     Completed Date:  2008-08-08     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8800135     Medline TA:  Genomics     Country:  United States    
Other Details:
Languages:  eng     Pagination:  335-46     Citation Subset:  IM    
Department of Membrane Biochemistry, Max-Planck-Institute of Biochemistry, Am Klopferspitz 18, D-82152 Martinsried, Germany.
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MeSH Terms
Amino Acid Sequence
Archaeal Proteins / chemistry,  genetics
Chromosomes, Archaeal
Genome, Archaeal*
Halobacterium salinarum / genetics*
Molecular Sequence Data
Sequence Homology, Amino Acid
Species Specificity
Reg. No./Substance:
0/Archaeal Proteins
Comment In:
Genomics. 2008 Jun;91(6):548-52; author reply 553-4   [PMID:  18538726 ]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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