| The evolution of fatty acid desaturases and cytochrome b5 in eukaryotes. | |
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MedLine Citation:
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PMID: 20146059 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Desaturases that introduce double bonds into the fatty acids are involved in the adaptation of membrane fluidity to changes in the environment. Besides, polyunsaturated fatty acids (PUFAs) are increasingly recognized as important pharmaceutical and nutraceutical compounds. To successfully engineer organisms with increased stress tolerance or the ability to synthesize valuable PUFAs, detailed knowledge about the complexity of the desaturase family as well as understanding of the coevolution of desaturases and their cytochrome b5 electron donors is needed. We have constructed phylogenies of several hundred desaturase sequences from animals, plants, fungi and bacteria and of the cytochrome b5 domains that are fused to some of these enzymes. The analysis demonstrates the existence of three major desaturase acyl-CoA groups that share few similarities. Our results indicate that the fusion of Delta(6)-desaturase-like enzymes with their cytochrome b5 electron donor was a single event that took place in the common ancestor of all eukaryotes. We also propose the Delta(6)-desaturase-like enzymes as the most probable donor of the cytochrome b5 domain found in fungal Delta(9)-desaturases and argue that the recombination most likely happened soon after the separation of the animal and fungal ancestors. These findings answer some of the previously unresolved questions and contribute to the quickly expanding field of research on desaturases. |
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Authors:
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Cene Gostincar; Martina Turk; Nina Gunde-Cimerman |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-02-10 |
Journal Detail:
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Title: The Journal of membrane biology Volume: 233 ISSN: 1432-1424 ISO Abbreviation: J. Membr. Biol. Publication Date: 2010 Feb |
Date Detail:
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Created Date: 2010-02-25 Completed Date: 2010-05-20 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0211301 Medline TA: J Membr Biol Country: United States |
Other Details:
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Languages: eng Pagination: 63-72 Citation Subset: IM |
Affiliation:
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Department of Biology, University of Ljubljana, Slovenia. cene.gostincar@bf.uni-lj.si |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acid Sequence Animals Computational Biology Cytochromes b5 / chemistry, classification*, genetics Eukaryota / enzymology* Fatty Acid Desaturases / chemistry, classification*, genetics Fatty Acids, Unsaturated / metabolism Molecular Sequence Data Phylogeny* Sequence Homology, Amino Acid |
| Chemical | |
Reg. No./Substance:
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0/Fatty Acids, Unsaturated; 9035-39-6/Cytochromes b5; EC 1.14.19.-/Fatty Acid Desaturases |
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