Document Detail


Evoked thalamic neuronal activity following DRG application of two nucleus pulposus derived cell populations: an experimental study in rats.
MedLine Citation:
PMID:  23341046     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
PURPOSE: To investigate the effects on evoked thalamic neuronal activity of application of notochordal cells and chondrocyte-like cells derived from nucleus pulposus (NP) onto a dorsal root ganglion (DRG) and to compare these effects with a previously reported increased thalamic activity induced by NP. METHODS: Nucleus pulposus was harvested from tail discs of adult rats and the disc cells were separated into two cell populations, notochordal cells and chondrocyte-like cells. The two cell populations were applied separately, or in combination, to the L4 DRG of anaesthetised female Sprague-Dawley rats during acute electrophysiological experiments. In control experiments, cell suspension medium was applied on the DRG. Recordings from the contralateral thalamus were sampled for 40 min while electrically stimulating the ipsilateral sciatic nerve at above Aδ-fibre thresholds. RESULTS: Application of notochordal cells resulted in a decrease in evoked thalamic activity within 10 min while chondrocyte-like cells did not induce any changes during the 40 min of recording. The difference in evoked thalamic activity 40 min after notochordal and chondrocyte-like cell application, respectively, was statistically significant. Neither an increased concentration of chondrocyte-like cells alone nor a combination of the two cell populations induced any changes in thalamic activity. CONCLUSIONS: Separate exposure of the DRG to the two NP-derived cell populations induced different effects on evoked thalamic activity, but none of the tested cell samples induced an increase in neuronal activity similar to that previously observed with NP. This indicates a high complexity of the interaction between NP and nervous tissue.
Authors:
E Nilsson; K Larsson; B Rydevik; H Brisby; I Hammar
Related Documents :
23032076 - Regulation of the cell proliferation and migration as extra-pituitary functions of gnrh.
23145796 - Non-endocytic delivery of functional engineered nanoparticles into the cytoplasm of liv...
23994576 - α-tocopherol administration blocks adaptive changes on cell nadh/nad(+) redox state an...
24005866 - Ziyuglycoside ii inhibits the growth of human breast carcinoma mda-mb-435 cells via cel...
21058906 - Sod2-mediated effects induced by wr1065 and low-dose ionizing radiation on micronucleus...
23032076 - Regulation of the cell proliferation and migration as extra-pituitary functions of gnrh.
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2013-1-24
Journal Detail:
Title:  European spine journal : official publication of the European Spine Society, the European Spinal Deformity Society, and the European Section of the Cervical Spine Research Society     Volume:  -     ISSN:  1432-0932     ISO Abbreviation:  Eur Spine J     Publication Date:  2013 Jan 
Date Detail:
Created Date:  2013-1-23     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9301980     Medline TA:  Eur Spine J     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Affiliation:
Department of Physiology, Institute of Neuroscience and Physiology, University of Gothenburg, PO Box 432, 405 30, Gothenburg, Sweden, elin.nilsson@physiol.gu.se.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Comparison of scoliosis measurements based on three-dimensional vertebra vectors and conventional tw...
Next Document:  Antifungal Activity of Geldanamycin Alone or in Combination with Fluconazole Against Candida species...