Document Detail


Evidence for water-permeable channels in auditory hair cells in the leopard frog.
MedLine Citation:
PMID:  22940201     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Auditory hair cells in the amphibian papilla (APHCs) of the leopard frog, Rana pipiens pipiens, have a significantly higher permeability to water than that observed in mammalian hair cells. The insensitivity of water permeability in frog hair cells to extracellular mercury suggests that an amphibian homologue of the water channel aquaporin-4 (AQP4) may mediate water transport in these cells. Using immunocytochemistry, we show that an AQP4-like protein is found in APHCs. Rabbit anti-AQP4 antibody was used in multiple-immunohistochemical staining experiments along with AP hair cell and hair bundle markers in leopard frog and mouse tissue. AQP4 immunoreactivity was found in the basal and apical poles of the APHCs and shows uniform immunoreactivity. This study provides the first identification and localization of an AQP4-like protein in the amphibian inner ear. We also report a more direct measure of hyperosmotically-induced volume changes in APHCs that confirms previous findings. The presence of water channels in anuran APHCs constitutes a novel physiological difference between amphibian and mammalian hair cell structure and function.
Authors:
Mia E Miller; Arian K Nasiri; Peyman O Farhangi; Nasser A Farahbakhsh; Ivan A Lopez; Peter M Narins; Dwayne D Simmons
Publication Detail:
Type:  Journal Article     Date:  2012-08-24
Journal Detail:
Title:  Hearing research     Volume:  292     ISSN:  1878-5891     ISO Abbreviation:  Hear. Res.     Publication Date:  2012 Oct 
Date Detail:
Created Date:  2012-09-24     Completed Date:  2013-02-12     Revised Date:  2013-10-17    
Medline Journal Info:
Nlm Unique ID:  7900445     Medline TA:  Hear Res     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  64-70     Citation Subset:  IM    
Copyright Information:
Copyright © 2012 Elsevier B.V. All rights reserved.
Affiliation:
Department of Head and Neck Surgery, David Geffen School of Medicine, University of California, Los Angeles, CA 90995-1624, USA.
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MeSH Terms
Descriptor/Qualifier:
Amphibian Proteins / metabolism*
Animals
Aquaporin 4 / metabolism
Aquaporins / metabolism*
Cell Membrane Permeability*
Cell Size
Hair Cells, Auditory / metabolism*
Immunohistochemistry
Mice
Mice, Inbred C57BL
Microscopy, Confocal
Osmosis
Rana pipiens / metabolism*
Time Factors
Water / metabolism*
Grant Support
ID/Acronym/Agency:
R01 DC004086/DC/NIDCD NIH HHS
Chemical
Reg. No./Substance:
0/Amphibian Proteins; 0/Aqp4 protein, mouse; 0/Aquaporin 4; 0/Aquaporins; 7732-18-5/Water
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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