Document Detail


Evidence of two functionally distinct ornithine decarboxylation systems in lactic acid bacteria.
MedLine Citation:
PMID:  22247134     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Biogenic amines are low-molecular-weight organic bases whose presence in food can result in health problems. The biosynthesis of biogenic amines in fermented foods mostly proceeds through amino acid decarboxylation carried out by lactic acid bacteria (LAB), but not all systems leading to biogenic amine production by LAB have been thoroughly characterized. Here, putative ornithine decarboxylation pathways consisting of a putative ornithine decarboxylase and an amino acid transporter were identified in LAB by strain collection screening and database searches. The decarboxylases were produced in heterologous hosts and purified and characterized in vitro, whereas transporters were heterologously expressed in Lactococcus lactis and functionally characterized in vivo. Amino acid decarboxylation by whole cells of the original hosts was determined as well. We concluded that two distinct types of ornithine decarboxylation systems exist in LAB. One is composed of an ornithine decarboxylase coupled to an ornithine/putrescine transmembrane exchanger. Their combined activities results in the extracellular release of putrescine. This typical amino acid decarboxylation system is present in only a few LAB strains and may contribute to metabolic energy production and/or pH homeostasis. The second system is widespread among LAB. It is composed of a decarboxylase active on ornithine and l-2,4-diaminobutyric acid (DABA) and a transporter that mediates unidirectional transport of ornithine into the cytoplasm. Diamines that result from this second system are retained within the cytosol.
Authors:
Andrea Romano; Hein Trip; Aline Lonvaud-Funel; Juke S Lolkema; Patrick M Lucas
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2012-01-13
Journal Detail:
Title:  Applied and environmental microbiology     Volume:  78     ISSN:  1098-5336     ISO Abbreviation:  Appl. Environ. Microbiol.     Publication Date:  2012 Mar 
Date Detail:
Created Date:  2012-02-23     Completed Date:  2012-05-16     Revised Date:  2013-06-26    
Medline Journal Info:
Nlm Unique ID:  7605801     Medline TA:  Appl Environ Microbiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1953-61     Citation Subset:  IM    
Affiliation:
University of Bordeaux, ISVV, Villenave d’Ornon, France.
Data Bank Information
Bank Name/Acc. No.:
GENBANK/JN120479
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MeSH Terms
Descriptor/Qualifier:
Amino Acid Transport Systems / genetics,  metabolism
Cluster Analysis
DNA, Bacterial / chemistry,  genetics
Decarboxylation
Kinetics
Lactobacillales / enzymology*,  genetics,  metabolism*
Molecular Sequence Data
Ornithine / metabolism*
Ornithine Decarboxylase / genetics,  isolation & purification,  metabolism
Phylogeny
Sequence Analysis, DNA
Sequence Homology
Chemical
Reg. No./Substance:
0/Amino Acid Transport Systems; 0/DNA, Bacterial; 7006-33-9/Ornithine; EC 4.1.1.17/Ornithine Decarboxylase
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