| EVIDENCE FOR A TRICARBOXYLIC ACID CYCLE IN MYCOPLASMA HOMINIS. | |
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MedLine Citation:
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PMID: 14240945 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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VanDemark, P. J. (Cornell University, Ithaca, N.Y.), and P. F. Smith. Evidence for a tricarboxylic acid cycle in Mycoplasma hominis. J. Bacteriol. 88:1602-1607. 1964.-Resting cells of acetate-grown Mycoplasma hominis strain 07 oxidized the various intermediates of the tricarboxylic and glyoxylate cycles, with the exception of sodium citrate and glyoxylate. Extracts of these cells possessed isocitric dehydrogenase, isocitratase, alpha-ketoglutaric dehydrogenase, succinic dehydrogenase, fumarase, malic dehydrogenase, citratase, and acetyl coenzyme A kinase activities. With the assay conditions employed, condensing enzyme, malate synthetase, and phosphotransacetylase activities were negligible. Incubation of sodium acetate-2-C(14) with the various intermediates of the tricarboxylic acid cycle in the presence of cell-free extracts resulted in exchange of the isotope with these compounds as well as the formation of other labeled intermediates of the tricarboxylic acid cycle. Oxidation of sodium acetate-2-C(14) alone resulted in the formation of labeled succinate, fumarate, and malate. |
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Authors:
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P J VANDEMARK; P F SMITH |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Journal of bacteriology Volume: 88 ISSN: 0021-9193 ISO Abbreviation: J. Bacteriol. Publication Date: 1964 Dec |
Date Detail:
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Created Date: 1965-04-01 Completed Date: 1996-12-01 Revised Date: 2009-11-18 |
Medline Journal Info:
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Nlm Unique ID: 2985120R Medline TA: J Bacteriol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 1602-7 Citation Subset: OM |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Carbon Isotopes* Citric Acid Cycle* Enzymes* Fatty Acids* Metabolism* Mycoplasma* Research* |
| Chemical | |
Reg. No./Substance:
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0/Carbon Isotopes; 0/Enzymes; 0/Fatty Acids |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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