Document Detail


Evidence that sucrose loaded into the phloem of a poplar leaf is used directly by sucrose synthase associated with various beta-glucan synthases in the stem.
MedLine Citation:
PMID:  14988476     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Sucrose (Suc) synthase (SuSy) is believed to function in channeling UDP-Glc from Suc to various beta-glucan synthases. We produced transgenic poplars (Populus alba) overexpressing a mutant form (S11E) of mung bean (Vigna radiata) SuSy, which appeared in part in the microsomal membranes of the stems. Expression of SuSy in these membranes enhanced the incorporation of radioactive Suc into cellulose, together with the metabolic recycling of fructose (Fru), when dual-labeled Suc was fed directly into the phloem of the leaf. This overexpression also enhanced the direct incorporation of the glucosyl moiety of Suc into the glucan backbone of xyloglucan and increased recycling of Fru, although the Fru recycling system for cellulose synthesis at the plasma membrane might differ from that for xyloglucan synthesis in the Golgi network. These findings suggest that some of the Suc loaded into the phloem of a poplar leaf is used directly by SuSys associated with xyloglucan and cellulose synthases in the stem. This may be a key function of SuSy because the high-energy bond between the Glc and Fru moieties of Suc is conserved and used for polysaccharide syntheses in this sink tissue.
Authors:
Teruko Konishi; Yasunori Ohmiya; Takahisa Hayashi
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Publication Detail:
Type:  Journal Article     Date:  2004-02-26
Journal Detail:
Title:  Plant physiology     Volume:  134     ISSN:  0032-0889     ISO Abbreviation:  Plant Physiol.     Publication Date:  2004 Mar 
Date Detail:
Created Date:  2004-03-15     Completed Date:  2004-05-27     Revised Date:  2010-09-20    
Medline Journal Info:
Nlm Unique ID:  0401224     Medline TA:  Plant Physiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1146-52     Citation Subset:  IM    
Affiliation:
Wood Research Institute, Kyoto University, Uji, Kyoto 611-0011, Japan.
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MeSH Terms
Descriptor/Qualifier:
Base Sequence
DNA, Plant / genetics
Fabaceae / enzymology,  genetics
Genes, Plant
Glucans / metabolism
Glucosyltransferases / genetics,  metabolism*
Mutation
Plant Leaves / metabolism
Plants, Genetically Modified
Populus / enzymology,  genetics,  metabolism*
Sucrose / metabolism*
Xylans / metabolism
Chemical
Reg. No./Substance:
0/DNA, Plant; 0/Glucans; 0/Xylans; 37294-28-3/xyloglucan; 57-50-1/Sucrose; EC 2.4.1.-/Glucosyltransferases; EC 2.4.1.-/glucan synthase; EC 2.4.1.13/sucrose synthase
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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