Document Detail

Evidence that serum NTx (collagen-type I N-telopeptides) can act as an immunochemical marker of bone resorption.
MedLine Citation:
PMID:  9365389     Owner:  NLM     Status:  MEDLINE    
Previous studies have shown that immunoassay of urinary NTx (cross-linked N-telopeptides of type I collagen) provides a responsive index of human bone resorption. Here we report by a sensitive immunoassay that NTx is present in serum and is suppressed appropriately in patients with Paget disease of bone by bisphosphonate antiresorptive therapy. The monoclonal antibody (1H11) developed against urinary NTx was applied in a sensitive chemiluminescence format. Results for human serum and urine showed parallel inhibition curves. The NTx concentrations in paired serum and urine samples from individual patients correlated well when urinary concentrations were normalized to creatinine concentrations (in premenopausal and postmenopausal women and Paget disease patients, r = 0.90, n = 60). The percentage of NTx suppression from baseline values for Paget disease patients on bisphosphonate therapy was similar for serum and urine. Blood samples drawn from bone marrow at the site of Pagetic lesions in three patients with active disease had as much as 10-fold higher concentrations of NTx than did peripheral blood samples drawn at the same time. The latter finding is consistent with other evidence showing that immunoreactive NTx originates directly during the proteolytic cleavage of bone collagen by osteoclasts rather than, e.g., by degradative processes occurring later in the liver and kidney.
J D Clemens; M V Herrick; F R Singer; D R Eyre
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Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  Clinical chemistry     Volume:  43     ISSN:  0009-9147     ISO Abbreviation:  Clin. Chem.     Publication Date:  1997 Nov 
Date Detail:
Created Date:  1997-12-08     Completed Date:  1997-12-08     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9421549     Medline TA:  Clin Chem     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  2058-63     Citation Subset:  IM    
Ostex International, Inc., Seattle, WA 98134, USA.
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MeSH Terms
Antibodies, Monoclonal / immunology
Biological Markers / blood
Bone Resorption / diagnosis*,  drug therapy,  urine
Collagen / blood*,  immunology
Collagen Type I
Diphosphonates / therapeutic use
Luminescent Measurements
Middle Aged
Osteitis Deformans / diagnosis,  drug therapy,  urine
Peptides / blood*,  immunology
Postmenopause / blood,  urine
Premenopause / blood,  urine
Reproducibility of Results
Reg. No./Substance:
0/Antibodies, Monoclonal; 0/Biological Markers; 0/Collagen Type I; 0/Diphosphonates; 0/Peptides; 0/collagen type I trimeric cross-linked peptide; 9007-34-5/Collagen

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