Document Detail

Evidence that a phosphatidylinositol 3,4,5-trisphosphate-binding protein can function in nucleus.
MedLine Citation:
PMID:  9933577     Owner:  NLM     Status:  MEDLINE    
PIP3BP is a phosphatidylinositol 3,4,5-trisphosphate-binding protein (PIP3BP) abundant in brain, containing a zinc finger motif and two pleckstrin homology (PH) domains. Staining of rat brain cells with anti-PIP3BP antibody and determination of localization of PIP3BP fused to the green fluorescent protein (GFP-PIP3BP) revealed that PIP3BP was targeted to the nucleus. Targeting was dependent on a putative nuclear localization signal in PIP3BP. Generation of PIP3 in the nucleus was detected in H2O2-treated 293T cells, nerve growth factor (NGF)-treated PC12 cells, and platelet-derived growth factor (PDGF)-treated NIH 3T3 cells. Translocation of phosphatidylinositol 3-kinase (PI 3-kinase) to the nucleus and enhanced activity of PI 3-kinase in the nucleus fraction were observed after H2O2 treatment of 293T cells, suggesting that PI 3-kinase can be activated in the nucleus as well as in the membrane after appropriate stimulation of the cells. Co-expression of the constitutively active PI 3-kinase with PIP3BP resulted in exportation of the protein from the nucleus to the cytoplasm, suggesting that PIP3BP can function as a PIP3-binding protein in the intact cells. These results imply that there may be an unknown function of PI 3-kinase in the nucleus.
K Tanaka; K Horiguchi; T Yoshida; M Takeda; H Fujisawa; K Takeuchi; M Umeda; S Kato; S Ihara; S Nagata; Y Fukui
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Journal of biological chemistry     Volume:  274     ISSN:  0021-9258     ISO Abbreviation:  J. Biol. Chem.     Publication Date:  1999 Feb 
Date Detail:
Created Date:  1999-03-11     Completed Date:  1999-03-11     Revised Date:  2011-09-28    
Medline Journal Info:
Nlm Unique ID:  2985121R     Medline TA:  J Biol Chem     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  3919-22     Citation Subset:  IM    
Laboratory of Biological Chemistry, Department of Applied Biological Chemistry, Graduate School of Agriculture and Life Science, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-0032, Japan.
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MeSH Terms
3T3 Cells
Adaptor Proteins, Signal Transducing
Biological Transport
Blood Proteins / chemistry
Brain Chemistry
COS Cells
Carrier Proteins / metabolism*
Cell Nucleus / metabolism*
Enzyme Activation
In Situ Hybridization
Neurons / metabolism
PC12 Cells
Phosphatidylinositol 3-Kinases / metabolism*
Phosphatidylinositol Phosphates / metabolism*
Sequence Homology, Amino Acid
Zinc Fingers
Reg. No./Substance:
0/Adaptor Proteins, Signal Transducing; 0/Blood Proteins; 0/Carrier Proteins; 0/Phosphatidylinositol Phosphates; 0/Phosphoproteins; 0/phosphatidylinositol 3,4,5-triphosphate; 0/platelet protein P47; EC 2.7.1.-/Phosphatidylinositol 3-Kinases

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