| Evidence that neutral sphingomyelinase of cultured murine neuroblastoma cells is oriented externally on the plasma membrane. | |
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MedLine Citation:
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PMID: 6091759 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The activity of the neutral, Mg2+-stimulated sphingomyelinase of cultured neuroblastoma cells (N1E-115) is enriched in the plasma membrane fraction and is reduced following treatment of intact or broken cells with trypsin, alpha-chymotrypsin, papain, and protease. Two protease-sensitive enzymes of the cell interior (lactate dehydrogenase and NADPH-cytochrome c reductase) are not affected by protease treatment of intact cells. These results indicate that the neutral, Mg2+-stimulated sphingomyelinase is oriented externally on the plasma membrane of the cultured neuroblastoma cell. |
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Authors:
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D V Das; H W Cook; M W Spence |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Biochimica et biophysica acta Volume: 777 ISSN: 0006-3002 ISO Abbreviation: Biochim. Biophys. Acta Publication Date: 1984 Nov |
Date Detail:
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Created Date: 1984-12-05 Completed Date: 1984-12-05 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0217513 Medline TA: Biochim Biophys Acta Country: NETHERLANDS |
Other Details:
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Languages: eng Pagination: 339-42 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cell Line Cell Membrane / enzymology* Chymotrypsin / pharmacology L-Lactate Dehydrogenase / antagonists & inhibitors Magnesium / pharmacology Mice NADPH-Ferrihemoprotein Reductase / antagonists & inhibitors Neuroblastoma / enzymology* Papain / pharmacology Peptide Hydrolases / pharmacology Phosphoric Diester Hydrolases / metabolism* Sphingomyelin Phosphodiesterase / antagonists & inhibitors, metabolism* Trypsin / pharmacology |
| Chemical | |
Reg. No./Substance:
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7439-95-4/Magnesium; EC 1.1.1.27/L-Lactate Dehydrogenase; EC 1.6.2.4/NADPH-Ferrihemoprotein Reductase; EC 3.1.4.-/Phosphoric Diester Hydrolases; EC 3.1.4.12/Sphingomyelin Phosphodiesterase; EC 3.4.-/Peptide Hydrolases; EC 3.4.21.1/Chymotrypsin; EC 3.4.21.4/Trypsin; EC 3.4.22.2/Papain |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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