Document Detail


Evidence that marginal zone B cells possess an enhanced secretory apparatus and exhibit superior secretory activity.
MedLine Citation:
PMID:  16951340     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Marginal zone B (MZB) cells are the first splenic B cells to initiate Ab secretion against polysaccharide-encapsulated Ags in vivo. This swift MZB cell response can be reproduced in vitro as LPS treatment induces Ab secretion in as little as 12 h. Conversely, in vitro LPS treatment of splenic follicular B (FOB) cells results in Ab secretion after 2-3 days. The basis for these distinct response kinetics is not understood. We performed ex vivo analysis of resting and LPS-stimulated murine MZB and FOB cells and found that MZB cells express higher levels of the LPS TLR complex RP105/MD-1 and respond to much lower concentrations of LPS than do FOB cells. Furthermore, increasing doses of LPS do not accelerate the kinetics by which FOB cells transition into Ab secretion. Ultrastructural analysis of resting cells demonstrated that rough endoplasmic reticulum is more abundant in MZB cells than in FOB cells. Additionally, RT-PCR and immunoblot analyses revealed that numerous endoplasmic reticulum resident chaperones and folding enzymes are expressed at greater levels in resting MZB cells than in resting FOB cells. Although both LPS-stimulated MZB and FOB cells increase expression of these factors, MZB cells exhibit a more rapid increase that correlates with accelerated kinetics of Ab secretion and higher per cell output of secreted IgM. These data indicate that MZB cells are equipped for exquisite sensitivity to bacterial components like LPS and poised for rapid, robust Ab production, making MZB cells ideally suited as frontline defenders in humoral immunity.
Authors:
Kathryn E Gunn; Joseph W Brewer
Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, N.I.H., Extramural    
Journal Detail:
Title:  Journal of immunology (Baltimore, Md. : 1950)     Volume:  177     ISSN:  0022-1767     ISO Abbreviation:  J. Immunol.     Publication Date:  2006 Sep 
Date Detail:
Created Date:  2006-09-04     Completed Date:  2006-10-24     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  2985117R     Medline TA:  J Immunol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  3791-8     Citation Subset:  AIM; IM    
Affiliation:
Department of Microbiology and Immunology, Stritch School of Medicine, Loyola University Chicago, Maywood, IL 60153, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
B-Lymphocyte Subsets / immunology,  metabolism,  secretion*,  ultrastructure
Cells, Cultured
DNA-Binding Proteins / biosynthesis
Female
G0 Phase / immunology
Immunophenotyping
Lipopolysaccharides / pharmacology
Mice
Mice, Inbred C57BL
Nuclear Proteins / biosynthesis
Repressor Proteins / biosynthesis
Spleen / cytology,  immunology*,  secretion*,  ultrastructure
Toll-Like Receptors / biosynthesis
Transcription Factors / biosynthesis
Grant Support
ID/Acronym/Agency:
GM61970/GM/NIGMS NIH HHS; T32 AI007508/AI/NIAID NIH HHS
Chemical
Reg. No./Substance:
0/DNA-Binding Proteins; 0/Lipopolysaccharides; 0/Nuclear Proteins; 0/Prdm1 protein, mouse; 0/Repressor Proteins; 0/Toll-Like Receptors; 0/Transcription Factors; 0/regulatory factor X transcription factors

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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