Document Detail


Evidence that the major metabolites accumulating in hyperornithinemia-hyperammonemia-homocitrullinuria syndrome induce oxidative stress in brain of young rats.
MedLine Citation:
PMID:  19683047     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Ornithine and homocitrulline are the major metabolites accumulating in hyperornithinemia-hyperammonemia-homocitrullinuria syndrome, a genetic disorder characterized by neurological regression whose pathogenesis is still not understood. The present work investigated the in vitro effects of ornithine and homocitrulline on important parameters of oxidative stress in cerebral cortex from young rats. Ornithine significantly increased chemiluminescence and thiobarbituric acid-reactive substances levels, indicators of lipid peroxidation, while homocitrulline only augmented chemiluminescence values. Furthermore, ornithine-induced increase of thiobarbituric acid-reactive substances levels was attenuated (melatonin and reduced glutathione) or totally prevented (alpha-tocopherol) by free radical scavengers, suggesting that reactive species were involved in the lipid oxidative damage. We also observed that ornithine and homocitrulline significantly decreased the tissue antioxidant defenses, determined by reduced glutathione concentrations, the major non-enzymatic antioxidant defense found in the brain. Homocitrulline reduction of glutathione levels was completely prevented by melatonin and alpha-tocopherol, whereas ornithine-induced decrease of glutathione levels was only attenuated by these free radical scavengers. Ornithine and homocitrulline also induced protein oxidative damage, increasing carbonyl formation and sulfhydryl oxidation. In contrast, these amino acids did not affect nitric oxide production, indicating that nitrogen reactive species were not implicated in the lipid and oxidative damage provoked by ornithine and homocitrulline. Therefore, it is presumed that the major metabolites accumulating in hyperornithinemia-hyperammonemia-homocitrullinuria syndrome elicit oxidative stress and that this pathomechanism may possibly be involved in the brain damage found in patients affected by this disorder.
Authors:
Alexandre Umpierrez Amaral; Guilhian Leipnitz; Carolina Gonçalves Fernandes; Bianca Seminotti; Angela Zanatta; Carolina Maso Viegas; Carlos Severo Dutra-Filho; Moacir Wajner
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-08-13
Journal Detail:
Title:  International journal of developmental neuroscience : the official journal of the International Society for Developmental Neuroscience     Volume:  27     ISSN:  1873-474X     ISO Abbreviation:  Int. J. Dev. Neurosci.     Publication Date:  2009 Nov 
Date Detail:
Created Date:  2009-09-29     Completed Date:  2009-12-01     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8401784     Medline TA:  Int J Dev Neurosci     Country:  England    
Other Details:
Languages:  eng     Pagination:  635-41     Citation Subset:  IM    
Affiliation:
Departamento de Bioquímica, Instituto de Ciências Básicas da Saúde, Universidade Federal de Rio Grande do Sul, Porto Alegre, RS, Brazil.
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MeSH Terms
Descriptor/Qualifier:
Animals
Antioxidants / metabolism
Brain / metabolism*
Citrulline / analogs & derivatives*,  urine
Glutathione / metabolism
Humans
Hyperammonemia / metabolism*
Lipid Peroxidation
Male
Nitric Oxide / metabolism
Ornithine / metabolism*
Oxidation-Reduction
Oxidative Stress*
Rats
Rats, Wistar
Syndrome
Thiobarbituric Acid Reactive Substances / metabolism
Chemical
Reg. No./Substance:
0/Antioxidants; 0/Thiobarbituric Acid Reactive Substances; 10102-43-9/Nitric Oxide; 1190-49-4/homocitrulline; 372-75-8/Citrulline; 70-18-8/Glutathione; 7006-33-9/Ornithine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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