Document Detail


Evidence that kinetochore fibre microtubules shorten predominantly at the pole in anaphase flea-beetle spermatocytes.
MedLine Citation:
PMID:  10780704     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Kinetochore spindle fibres in flea-beetle metaphase primary spermatocytes have two regions with distinct morphologies. As seen after staining with antibodies against tubulin, the kinetochore microtubules are tightly bundled in the 5 microm closest to the kinetochore but they splay out in the region closest to the pole. This morphology persists throughout anaphase. This distinct morphology allows one to deduce the site where kinetochore microtubules depolymerise during anaphase. During poleward movement of the autosomes in anaphase, the bundled region shortens by about 0.25 microm for each 1 microm the chromosome moves poleward; this suggests that, during anaphase, 75% of the kinetochore microtubule shortening occurs at the pole end. Sex chromosomes in metaphase cells are separated from the autosomes and do not move poleward at the same time as the autosomes: they are reported to move poleward when the autosomes are in metaphase, to stop part way to the poles, and to move poleward again as the autosomes do (Virkki 1970). Kinetochore microtubules of the sex chromosomes also have bundled and splayed regions; measurements of those regions suggest that these chromosomes may move poleward before the autosomes enter anaphase, but not afterwards.
Authors:
A Forer; P J Wilson
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology     Volume:  8     ISSN:  0967-3849     ISO Abbreviation:  Chromosome Res.     Publication Date:  2000  
Date Detail:
Created Date:  2000-06-23     Completed Date:  2000-06-23     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9313452     Medline TA:  Chromosome Res     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  151-63     Citation Subset:  IM    
Affiliation:
Biology Department, York University, North York, Ontario, Canada. aforer@yorku.ca
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MeSH Terms
Descriptor/Qualifier:
Anaphase*
Animals
Beetles / genetics*
Kinetochores*
Male
Metaphase
Microscopy, Confocal
Microscopy, Phase-Contrast
Microtubules / metabolism*
Sex Chromosomes
Spermatocytes*
Tubulin / metabolism
Chemical
Reg. No./Substance:
0/Tubulin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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