Document Detail


Evidence that the enterotoxin gene can be episomal in Clostridium perfringens isolates associated with non-food-borne human gastrointestinal diseases.
MedLine Citation:
PMID:  9431915     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Clostridium perfringens enterotoxin (CPE) is responsible for the diarrheal and cramping symptoms of human C. perfringens type A food poisoning. CPE-producing C. perfringens isolates have also recently been associated with several non-food-borne human gastrointestinal (GI) illnesses, including antibiotic-associated diarrhea and sporadic diarrhea. The current study has used restriction fragment length polymorphism (RFLP) and pulsed-field gel electrophoresis (PFGE) analyses to compare the genotypes of 43 cpe-positive C. perfringens isolates obtained from diverse sources. All North American and European food-poisoning isolates examined in this study were found to carry a chromosomal cpe, while all non-food-borne human GI disease isolates characterized in this study were determined to carry their cpe on an episome. Collectively, these results provide the first evidence that distinct subpopulations of cpe-positive C. perfringens isolates may be responsible for C. perfringens type A food poisoning versus CPE-associated non-food-borne human GI diseases. If these putative associations are confirmed in additional surveys, cpe RFLP and PFGE genotyping assays may facilitate the differential diagnosis of food-borne versus non-food-borne CPE-associated human GI illnesses and may also be useful epidemiologic tools for identifying reservoirs or transmission mechanisms for the subpopulations of cpe-positive isolates specifically responsible for CPE-associated food-borne versus non-food-borne human GI diseases.
Authors:
R E Collie; B A McClane
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Journal of clinical microbiology     Volume:  36     ISSN:  0095-1137     ISO Abbreviation:  J. Clin. Microbiol.     Publication Date:  1998 Jan 
Date Detail:
Created Date:  1998-02-12     Completed Date:  1998-02-12     Revised Date:  2013-06-11    
Medline Journal Info:
Nlm Unique ID:  7505564     Medline TA:  J Clin Microbiol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  30-6     Citation Subset:  IM    
Affiliation:
Department of Molecular Genetics and Biochemistry, School of Medicine, University of Pittsburgh, Pennsylvania 15261, USA.
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MeSH Terms
Descriptor/Qualifier:
Chromosome Mapping
Clostridium perfringens / genetics*,  pathogenicity
Electrophoresis, Gel, Pulsed-Field
Enterotoxins / genetics*
Humans
Plasmids*
Polymorphism, Restriction Fragment Length
Grant Support
ID/Acronym/Agency:
AI 19844-15/AI/NIAID NIH HHS
Chemical
Reg. No./Substance:
0/Enterotoxins; 0/enterotoxin, Clostridium
Comments/Corrections

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