| Evidence that ceramide selectively inhibits protein kinase C-alpha translocation and modulates bradykinin activation of phospholipase D. | |
| | |
MedLine Citation:
|
PMID: 7890607 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Sphingomyelinase (SMase) treatment (0.1 unit/ml for up to 30 min) of mouse epidermal (HEL-37) or human skin fibroblast (SF 3155) cells preincubated with [3H]serine to label the sphingomyelin pool caused the accumulation of labeled ceramide but not sphingosine or ceramide 1-phosphate. Incubation of HEL-37 cells with dioctanoylglycerol (diC8) or SF 3155 cells with bradykinin caused translocation of calcium/phosphatidylserine-dependent protein kinase C (PKC) activity to particulate material. In both cell lines the translocation was blocked by SMase treatment of the cells or by incubation with the cell-permeable ceramide analogue N-acetylsphingosine (C2-Cer). Western blot analysis indicated that treatment of HEL-37 cells with diC8 or SF 3155 cells with bradykinin resulted in the translocation of both PKC-alpha and PKC-espilon to particulate material. Treatment with SMase or C2-Cer specifically blocked the translocation of PKC-alpha but not that of PKC-epsilon. Pretreatment of cells with SMase or C2-Cer also inhibited the activation of phospholipase D activity induced by either diC8 (HEL-37 cells) or bradykinin (SF 3155 cells). The data provide strong evidence that ceramide can negatively regulate the translocation of PKC-alpha but not PKC-epsilon and further suggest that PKC-alpha may be involved in regulating phospholipase D activity. |
| | |
Authors:
|
M J Jones; A W Murray |
Related Documents
:
|
3986807 - Cutaneous changes during prolonged application of 12-o-tetradecanoylphorbol-13-acetate ... 10726207 - Expression level of inositol trisphosphate and inositol tetrakisphosphate receptors and... 2125997 - Tumor-promoting phorbol ester induces alterations of sialidase and sialyltransferase ac... 22161117 - Genotoxic effect of ciprofloxacin during photolytic decomposition monitored by the in v... 6543557 - Rhythmic changes of human female uroepithelial squamous cells during menstrual cycle. t... 8722687 - Germ cell-specific dna and rna binding proteins p48/52 are expressed at specific stages... |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: The Journal of biological chemistry Volume: 270 ISSN: 0021-9258 ISO Abbreviation: J. Biol. Chem. Publication Date: 1995 Mar |
Date Detail:
|
Created Date: 1995-04-14 Completed Date: 1995-04-14 Revised Date: 2007-11-15 |
Medline Journal Info:
|
Nlm Unique ID: 2985121R Medline TA: J Biol Chem Country: UNITED STATES |
Other Details:
|
Languages: eng Pagination: 5007-13 Citation Subset: IM |
Affiliation:
|
School of Biological Sciences, Faculty of Science and Engineering, Flinders University, Adelaide, Australia. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Blotting, Western Bradykinin / pharmacology* Cell Line Ceramides / physiology* Enzyme Activation Epidermis Fibroblasts Humans Inositol Phosphates / metabolism Isoenzymes / antagonists & inhibitors, metabolism* Kinetics Mice Phospholipase D / metabolism* Protein Kinase C / antagonists & inhibitors, metabolism* Skin / enzymology Sphingomyelin Phosphodiesterase / pharmacology* Sphingosine / analogs & derivatives, pharmacology Time Factors |
| Chemical | |
Reg. No./Substance:
|
0/Ceramides; 0/Inositol Phosphates; 0/Isoenzymes; 0/N-acetylsphingosine; 123-78-4/Sphingosine; 58-82-2/Bradykinin; EC 2.7.11.13/Protein Kinase C; EC 3.1.4.12/Sphingomyelin Phosphodiesterase; EC 3.1.4.4/Phospholipase D |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Insulin receptor kinase activation releases a constraint maintaining the receptor on microvilli.
Next Document: CO binding kinetics of human cytochrome P450 3A4. Specific interaction of substrates with kineticall...