| Evidence that the antibiotic ciprofloxacin counteracts cyclosporine-dependent suppression of cytokine production. | |
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MedLine Citation:
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PMID: 8310519 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The fluoroquinolone antibiotic ciprofloxacin (cipro) has been reported to upregulate interleukin 2 and interferon-gamma production in lectin-stimulated lymphocytes. The aim of this study was to elucidate whether cipro and the immunosuppressive agent CsA have antagonistic action on cytokine synthesis. Accumulation of IL-2 and IFN-gamma protein and mRNA were analyzed in polyclonally (PHA or Con A) or alloantigen-stimulated human peripheral blood lymphocytes. CsA added simultaneously with PHA partially blocked cytokine synthesis. The present study also shows that cipro supplemented with CsA and PHA resulted in significant higher concentrations of IL-2 (up to 60 times) and IFN-gamma (4.3 times) as compared with PHA and CsA alone. Similar results were obtained with primary mixed lymphocyte reactions. In parallel, a greater amount of IL-2 and IFN-gamma mRNA was observed in lymphocytes incubated with both cipro and CsA as compared with CsA alone. Our results reveal that CsA-dependent inhibition of both IL-2 and IFN-gamma expression is counteracted by high concentrations of cipro. These findings may be of importance in clinical transplantation. |
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Authors:
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K Riesbeck; M Gullberg; A Forsgren |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Transplantation Volume: 57 ISSN: 0041-1337 ISO Abbreviation: Transplantation Publication Date: 1994 Jan |
Date Detail:
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Created Date: 1994-03-16 Completed Date: 1994-03-16 Revised Date: 2008-11-21 |
Medline Journal Info:
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Nlm Unique ID: 0132144 Medline TA: Transplantation Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 267-72 Citation Subset: IM |
Affiliation:
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Department of Medical Microbiology, Lund University, Malmö General Hospital, Umeå, Sweden. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Blotting, Northern Cells, Cultured Ciprofloxacin / pharmacology* Cyclosporine / antagonists & inhibitors*, pharmacology DNA Probes Drug Combinations Humans Interferon-gamma / biosynthesis*, genetics Interleukin-2 / biosynthesis*, genetics Lectins Lymphocyte Activation Lymphocytes / cytology, drug effects* RNA, Messenger / metabolism |
| Chemical | |
Reg. No./Substance:
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0/DNA Probes; 0/Drug Combinations; 0/Interleukin-2; 0/Lectins; 0/RNA, Messenger; 59865-13-3/Cyclosporine; 82115-62-6/Interferon-gamma; 85721-33-1/Ciprofloxacin |
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