Document Detail

Evidence that the anti-obesity effect of conjugated linoleic acid is independent of effects on stearoyl-CoA desaturase1 expression and enzyme activity.
MedLine Citation:
PMID:  14975733     Owner:  NLM     Status:  MEDLINE    
The trans-10,cis-12 isomer of conjugated linoleic acid (CLA) reduces body fat gain in animals and inhibits stearoyl-CoA desaturase (SCD) activity in 3T3-L1 adipocytes. To test whether CLA's body fat reduction is mediated by SCD1, wild-type and SCD1-null mice were fed diet supplemented with 0.2% trans-10,cis-12 (t10c12) CLA for 4 weeks. The t10c12 CLA-supplemented diet significantly reduced body fat mass in both wild type and SCD1-null mice. Similarly, t10c12 CLA diet decreased blood triglyceride and free fatty acid levels regardless of SCD1 genotypes. Mice fed t10c12 CLA exhibited increased mRNA expression of fatty acid synthase and uncoupling protein 2 in both genotypes. Taken together, the effects of t10c12 CLA on reduction of body fat gain, blood parameters, and mRNA expression in both SCD1-null mice and wild-type mice were similar, indicating that the anti-obesity effect of t10c12 CLA may be independent of the effects of this CLA isomer on SCD1 gene expression and enzyme activity.
Kihwa Kang; Makoto Miyazaki; James M Ntambi; Michael W Pariza
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Biochemical and biophysical research communications     Volume:  315     ISSN:  0006-291X     ISO Abbreviation:  Biochem. Biophys. Res. Commun.     Publication Date:  2004 Mar 
Date Detail:
Created Date:  2004-02-20     Completed Date:  2004-03-30     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  0372516     Medline TA:  Biochem Biophys Res Commun     Country:  United States    
Other Details:
Languages:  eng     Pagination:  532-7     Citation Subset:  IM    
Department of Food Microbiology and Toxicology, Food Research Institute, University of Wisconsin-Madison, Madison, WI 53706, USA.
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MeSH Terms
3-Hydroxybutyric Acid / blood
Adipose Tissue / chemistry,  metabolism
Animal Feed
Anti-Obesity Agents / chemistry*,  pharmacology*
Blood Glucose / analysis
Body Weight / drug effects
Cholesterol / blood
Fats / analysis
Fatty Acids / analysis,  chemistry
Gene Expression
Linoleic Acid / chemistry*,  pharmacology*
Liver / chemistry
Mice, Knockout
Muscles / chemistry
RNA, Messenger / biosynthesis
Stearoyl-CoA Desaturase / deficiency,  genetics,  metabolism*
Triglycerides / blood
Reg. No./Substance:
0/Anti-Obesity Agents; 0/Blood Glucose; 0/Fats; 0/Fatty Acids; 0/RNA, Messenger; 0/Triglycerides; 2197-37-7/Linoleic Acid; 300-85-6/3-Hydroxybutyric Acid; 57-88-5/Cholesterol; EC Desaturase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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