Document Detail


Evidence that aminoguanidine inhibits endotoxin-induced bacterial translocation.
MedLine Citation:
PMID:  9718006     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND: The role of inducible nitric oxide synthase (iNOS) in endotoxin-induced bacterial translocation was investigated by using its specific blocker aminoguanidine in 46 albino mice (25-35 g) allocated into four groups. METHODS: The first group received intraperitoneal saline (control; 0.9 per cent w v(-1) sodium chloride 1 ml kg(-1); n=6), the second group intraperitoneal endotoxin (Escherichia coli lipopolysaccharide 055:B5 20 mg kg(-1); n=19), the third group intraperitoneal aminoguanidine (20 mg kg(-1), 20 min before and 12 h after saline; n=6) and the fourth group both endotoxin and aminoguanidine intraperitoneally (n=15). Some 24 h later, the animals were anaesthetized with ether and blood samples were collected by cardiac puncture together with mesenteric lymph node (MLN), spleen and liver specimens under aseptic conditions. Specimens were then cultured to determine the presence of colony-forming units as an index of bacterial translocation. RESULTS: No bacterial growth was detected in samples from the first and third groups. Colony-forming bacteria were found in ten of 14 MLN samples, eight of 14 spleens, four of 14 livers and three of 14 peripheral blood samples in the second group, with E. coli being the predominant pathogen. In contrast, in the fourth group, colony-forming bacteria were found in only three of 14 MLN samples (P=0.02 versus the second group), three of 14 spleens and one of 14 liver specimens. None of the values in the fourth group was significantly different from those in the saline control group. CONCLUSION: The inhibition of iNOS during endotoxaemia by its specific blocker aminoguanidine attenuates the incidence of bacterial translocation in mice. These results may be exploited clinically for the prophylaxis and treatment of septic states.
Authors:
B Kavuklu; C Agalar; M O Guc; I Sayek
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The British journal of surgery     Volume:  85     ISSN:  0007-1323     ISO Abbreviation:  Br J Surg     Publication Date:  1998 Aug 
Date Detail:
Created Date:  1998-09-10     Completed Date:  1998-09-10     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0372553     Medline TA:  Br J Surg     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  1103-6     Citation Subset:  AIM; IM    
Affiliation:
Department of General Surgery, Faculty of Medicine, Hacettepe University, Ankara, Turkey.
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MeSH Terms
Descriptor/Qualifier:
Animals
Bacterial Translocation / drug effects*
Endotoxemia / metabolism
Endotoxins / pharmacology*
Enzyme Inhibitors / pharmacology*
Escherichia coli / isolation & purification
Guanidines / pharmacology*
Mice
Nitric Oxide Synthase / antagonists & inhibitors*
Nitric Oxide Synthase Type II
Proteus mirabilis / isolation & purification
Chemical
Reg. No./Substance:
0/Endotoxins; 0/Enzyme Inhibitors; 0/Guanidines; 79-17-4/pimagedine; EC 1.14.13.39/Nitric Oxide Synthase; EC 1.14.13.39/Nitric Oxide Synthase Type II; EC 1.14.13.39/Nos2 protein, mouse

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