Document Detail

Evidence that Sry is expressed in pre-Sertoli cells and Sertoli and granulosa cells have a common precursor.
MedLine Citation:
PMID:  11784049     Owner:  NLM     Status:  MEDLINE    
The expression of Sry in the undifferentiated, bipotential genital ridges of mammalian XY fetuses initiates testis development and is hypothesized to do so by directing supporting cell precursors to develop as Sertoli cells and not as granulosa cells. To directly test this hypothesis, transgenic mice expressing EGFP under the control of the Sry promoter were produced. After establishing that the transgene was expressed in fetal gonads similarly to endogenous Sry, the spatial and temporal expression of the Sry-EGFP transgene was investigated in developing gonads by using confocal microscopy and immunofluorescent histochemistry. This analysis indicated: (1) Sry is first expressed in cells located centrally in the genital ridge and then later in cells located at the cranial and caudal poles, (2) Sry is expressed exclusively in pre-Sertoli cells in the urogenital ridge, and (3) Sertoli and granulosa cells develop from a common precursor. These results support the hypothesis that Sry initiates testis differentiation by directing the development of supporting cell precursors as Sertoli rather than granulosa cells. Furthermore, the Sry expression pattern explains the nonrandom distribution of testicular and ovarian tissue in mammalian ovotestes.
K H Albrecht; E M Eicher
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Developmental biology     Volume:  240     ISSN:  0012-1606     ISO Abbreviation:  Dev. Biol.     Publication Date:  2001 Dec 
Date Detail:
Created Date:  2002-01-10     Completed Date:  2002-01-24     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0372762     Medline TA:  Dev Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  92-107     Citation Subset:  IM    
The Jackson Laboratory, 600 Main Street, Bar Harbor, Maine 04609, USA.
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MeSH Terms
Base Sequence
DNA Primers
DNA-Binding Proteins / genetics,  metabolism*
Gene Expression Regulation, Developmental*
Granulosa Cells / metabolism*
Green Fluorescent Proteins
Luminescent Proteins / metabolism
Mice, Transgenic
Nuclear Proteins*
Reverse Transcriptase Polymerase Chain Reaction
Sertoli Cells / metabolism*
Sex-Determining Region Y Protein
Transcription Factors*
Grant Support
Reg. No./Substance:
0/DNA Primers; 0/DNA-Binding Proteins; 0/Luminescent Proteins; 0/Nuclear Proteins; 0/Sex-Determining Region Y Protein; 0/Sry protein, mouse; 0/Transcription Factors; 147336-22-9/Green Fluorescent Proteins

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