| Evidence that Dot-dependent and -independent factors isolate the Legionella pneumophila phagosome from the endocytic network in mouse macrophages. | |
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MedLine Citation:
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PMID: 11207624 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Legionella pneumophila survives within macrophages by evading phagosome-lysosome fusion. To determine whether L. pneumophila resides in an intermediate endosomal compartment or is isolated from the endosomal pathway and to investigate what bacterial factors contribute to establishment of its vacuole, we applied a series of fluorescence microscopy assays. The majority of vacuoles, aged 2.5 min to 4 h containing post-exponential phase (PE) L. pneumophila, appeared to be separate from the endosomal pathway, as judged by the absence of transferrin receptor, LAMP-1, cathepsin D and each of four fluorescent probes used to label the endocytic pathway either before or after infection. In contrast, more than 70% of phagosomes that contained Escherichia coli, polystyrene beads, or exponential phase (E) L. pneumophila matured to phagolysosomes, as judged by co-localization with LAMP-1, cathepsin D and fluorescent endosomal probes. Surprisingly, neither bacterial viability nor the putative Dot/Icm transport complex was absolutely required for vacuole isolation; although phagosomes containing either formalin-killed PE wild-type or live PE dotA or dotB mutant L. pneumophila rapidly accumulated LAMP-1, less than 20% acquired lysosomal cathepsin D or fluorescent endosomal probes. Therefore, a Dot-dependent factor(s) isolates the L. pneumophila phagosome from a LAMP-1-containing compartment, and a formalin-resistant Dot-independent activity inhibits vacuolar accumulation of endocytosed material and delivery to the degradative lysosomes. |
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Authors:
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A D Joshi; S Sturgill-Koszycki; M S Swanson |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Cellular microbiology Volume: 3 ISSN: 1462-5814 ISO Abbreviation: Cell. Microbiol. Publication Date: 2001 Feb |
Date Detail:
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Created Date: 2001-02-26 Completed Date: 2001-03-22 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 100883691 Medline TA: Cell Microbiol Country: England |
Other Details:
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Languages: eng Pagination: 99-114 Citation Subset: IM |
Affiliation:
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Department of Microbiology and Immunology, The University of Michigan Medical School, Ann Arbor 48109, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Antigens, CD / metabolism Biological Markers Cathepsin D / metabolism Endoscopes Female Legionella pneumophila / pathogenicity* Lysosome-Associated Membrane Glycoproteins Lysosomes Macrophages / microbiology* Membrane Glycoproteins / metabolism Mice Models, Biological Molecular Probes Phagosomes / microbiology* Receptors, Transferrin / metabolism Transport Vesicles / microbiology* |
| Grant Support | |
ID/Acronym/Agency:
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R29AI40694-01BM/AI/NIAID NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Antigens, CD; 0/Biological Markers; 0/Lysosome-Associated Membrane Glycoproteins; 0/Membrane Glycoproteins; 0/Molecular Probes; 0/Receptors, Transferrin; EC 3.4.23.5/Cathepsin D |
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