| Evidence for the synthesis of soluble peptidoglycan fragments by protoplasts of Streptococcus faecalis. | |
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MedLine Citation:
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PMID: 809417 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Growing protoplasts of Streptococcus faecalis 9790 were found to synthesize and excrete soluble peptidoglycan fragments. The presence of soluble peptidoglycan derivatives in culture supernatants was determined by (i) incorporation of three different radioactively labeled precursors (L-lysine, D-alanine, and acetate) into products which, after hen egg-white lysozyme hydrolysis, had the same KD values on gel filtration as muramidase hydrolysis products of isolated walls; (ii) inhibition of net synthesis of these products by cycloserine and vancomycin; and (iii) identification of disaccharide-peptide monomer using the beta-elimination reaction, gel filtration, and high-voltage paper electrophoresis. Under the conditions of these experiments the presence of newly synthesized, acid-precipitable (macromolecular) peptidoglycan was not detected. The predominance of monomer (70 to 80%) in lysozyme digests of peptidoglycan synthesized by protoplasts was in sharp contrast to digest of walls from intact streptococci which contain mostly peptide cross-linked products. Biosynthesis and release of relatively uncross-linked, soluble peptidoglycan fragments by protoplasts was related to the absence of suitable, preexisting acceptor wall. |
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Authors:
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R S Rosenthal; D Jungkind; L Daneo-Moore; G D Shockman |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Journal of bacteriology Volume: 124 ISSN: 0021-9193 ISO Abbreviation: J. Bacteriol. Publication Date: 1975 Oct |
Date Detail:
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Created Date: 1975-12-29 Completed Date: 1975-12-29 Revised Date: 2009-11-18 |
Medline Journal Info:
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Nlm Unique ID: 2985120R Medline TA: J Bacteriol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 398-409 Citation Subset: IM |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Acetates
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metabolism Alanine / metabolism Cycloserine / pharmacology Enterococcus faecalis / metabolism* Hydrolysis Lysine / metabolism Muramidase / metabolism Peptide Biosynthesis Peptidoglycan / biosynthesis* Pronase / metabolism Protoplasts / metabolism* Solubility Stereoisomerism Tetracycline / pharmacology Tritium Vancomycin / pharmacology |
| Chemical | |
Reg. No./Substance:
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0/Acetates; 0/Peptidoglycan; 10028-17-8/Tritium; 1404-90-6/Vancomycin; 56-41-7/Alanine; 56-87-1/Lysine; 60-54-8/Tetracycline; 68-41-7/Cycloserine; EC 3.2.1.17/Muramidase; EC 3.4.24.-/Pronase |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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