Document Detail


Evidence for the synthesis of soluble peptidoglycan fragments by protoplasts of Streptococcus faecalis.
MedLine Citation:
PMID:  809417     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Growing protoplasts of Streptococcus faecalis 9790 were found to synthesize and excrete soluble peptidoglycan fragments. The presence of soluble peptidoglycan derivatives in culture supernatants was determined by (i) incorporation of three different radioactively labeled precursors (L-lysine, D-alanine, and acetate) into products which, after hen egg-white lysozyme hydrolysis, had the same KD values on gel filtration as muramidase hydrolysis products of isolated walls; (ii) inhibition of net synthesis of these products by cycloserine and vancomycin; and (iii) identification of disaccharide-peptide monomer using the beta-elimination reaction, gel filtration, and high-voltage paper electrophoresis. Under the conditions of these experiments the presence of newly synthesized, acid-precipitable (macromolecular) peptidoglycan was not detected. The predominance of monomer (70 to 80%) in lysozyme digests of peptidoglycan synthesized by protoplasts was in sharp contrast to digest of walls from intact streptococci which contain mostly peptide cross-linked products. Biosynthesis and release of relatively uncross-linked, soluble peptidoglycan fragments by protoplasts was related to the absence of suitable, preexisting acceptor wall.
Authors:
R S Rosenthal; D Jungkind; L Daneo-Moore; G D Shockman
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Journal of bacteriology     Volume:  124     ISSN:  0021-9193     ISO Abbreviation:  J. Bacteriol.     Publication Date:  1975 Oct 
Date Detail:
Created Date:  1975-12-29     Completed Date:  1975-12-29     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  2985120R     Medline TA:  J Bacteriol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  398-409     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Acetates / metabolism
Alanine / metabolism
Cycloserine / pharmacology
Enterococcus faecalis / metabolism*
Hydrolysis
Lysine / metabolism
Muramidase / metabolism
Peptide Biosynthesis
Peptidoglycan / biosynthesis*
Pronase / metabolism
Protoplasts / metabolism*
Solubility
Stereoisomerism
Tetracycline / pharmacology
Tritium
Vancomycin / pharmacology
Chemical
Reg. No./Substance:
0/Acetates; 0/Peptidoglycan; 10028-17-8/Tritium; 1404-90-6/Vancomycin; 56-41-7/Alanine; 56-87-1/Lysine; 60-54-8/Tetracycline; 68-41-7/Cycloserine; EC 3.2.1.17/Muramidase; EC 3.4.24.-/Pronase
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