Document Detail

Evidence for synthesis and release of catecholamines by human amniotic epithelial cells.
MedLine Citation:
PMID:  9427302     Owner:  NLM     Status:  MEDLINE    
The present study investigated the presence, possible synthesis and release of catecholamines (CA) by human amniotic epithelial cells (HAEC) using HPLC with electrochemical detection. The presence of CA was indicated by the detection of norepinephrine (NE), dopamine (DA) and its metabolite 3,4-dihydroxyphenylacetic acid (DOPAC) in extracts of cultured HAEC. Incubation of HAE cells in medium supplemented with 1-tyrosine (CA precursor) and tetrahydrobiopterin (tyrosine hydroxylase cofactor) significantly increased the production of catecholamines, suggesting CA synthesis by HAEC. In contrast, pharmacological inhibition of tyrosine hydroxylase by alpha-methyl-p-tyrosine (MPT) significantly reduced CA production, further confirming CA synthesis by HAEC. Catecholamines were also detected in the cell incubation media, demonstrating the ability of HAEC to spontaneously secrete CA. Moreover, incubation of cells with 50 mM K+ for 10 min increased the amount of CA released into the medium. Additionally, the detection of DOPAC, a primary metabolite of DA, in HAEC strongly indicates that these cells contain DA metabolizing enzymes. The present results suggest that HAEC synthesize and release CA. These cells may be a possible candidate for transplantation therapy of neurodegenerative diseases such as Parkinson's disease and also may serve as a model to study the aspects of catecholaminergic activity.
M A Elwan; N Sakuragawa
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Neuroreport     Volume:  8     ISSN:  0959-4965     ISO Abbreviation:  Neuroreport     Publication Date:  1997 Nov 
Date Detail:
Created Date:  1998-02-26     Completed Date:  1998-02-26     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9100935     Medline TA:  Neuroreport     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  3435-8     Citation Subset:  IM    
Department of Inherited Metabolic Diseases, National Institute of Neuroscience, NCNP, Kodaira, Tokyo, Japan.
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MeSH Terms
3,4-Dihydroxyphenylacetic Acid / metabolism
Amnion / cytology,  metabolism*
Catecholamines / metabolism*
Cells, Cultured
Chromatography, High Pressure Liquid
Dopamine / metabolism
Epithelial Cells / cytology,  drug effects,  metabolism
Norepinephrine / metabolism
alpha-Methyltyrosine / pharmacology
Reg. No./Substance:
0/Catecholamines; 102-32-9/3,4-Dihydroxyphenylacetic Acid; 51-41-2/Norepinephrine; 658-48-0/alpha-Methyltyrosine

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