Document Detail


Evidence for a switch in the site of relaxin production from small theca-derived cells to large luteal cells during early pregnancy in the pig.
MedLine Citation:
PMID:  2644428     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The presence of immunoreactive relaxin was studied in corpora lutea of sows during the oestrous cycle and early pregnancy by immunohistochemistry and radioimmunoassay using three different anti-relaxin sera. Sections were immunostained using the peroxidase-anti-peroxidase or the immunogold-silver technique. Before Day 14, staining in corpora lutea from non-pregnant and pregnant animals was indistinguishable. With all antisera, no immunostaining was seen on Day 3, but was detected on Days 5-7 in cells from the theca interna. In non-pregnant animals, this immunostaining decreased and by Day 15 only an occasional large cell in the centre of the corpus luteum was stained. No staining was seen by Day 22. The relaxin content of corpora lutea measured by radioimmunoassay remained low throughout the luteal phase. In contrast, the amount of immunoreactive relaxin in corpora lutea rose dramatically (140-fold) between Days 11 and 14 of pregnancy and by Day 14 of pregnancy immunostaining was seen in the majority of large luteal cells. By Day 20 of pregnancy the concentrations of immunoreactive relaxin had further increased. Histochemical staining for alkaline phosphatase suggested that, while the relaxin-immunoreactive cells seen in the early luteal phase may be theca-derived, those during early pregnancy may be derived from the granulosa. The results are compatible with the suggestion that relaxin is produced by theca-derived cells during the early luteal phase and that between Days 11 and 14 there is a switch in the site of relaxin synthesis from theca-derived cells to granulosa-derived large luteal cells. In the absence of luteolysis, as during pregnancy, this switch is accompanied by a dramatic increase in relaxin synthesis.
Authors:
P A Denning-Kendall; S E Guldenaar; D C Wathes
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of reproduction and fertility     Volume:  85     ISSN:  0022-4251     ISO Abbreviation:  J. Reprod. Fertil.     Publication Date:  1989 Jan 
Date Detail:
Created Date:  1989-03-23     Completed Date:  1989-03-23     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0376367     Medline TA:  J Reprod Fertil     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  261-71     Citation Subset:  IM    
Affiliation:
Department of Anatomy, Medical School, University of Bristol, UK.
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MeSH Terms
Descriptor/Qualifier:
Animals
Corpus Luteum / metabolism*
Estrus / metabolism
Female
Granulosa Cells / metabolism
Immunoenzyme Techniques
Pregnancy
Pregnancy, Animal / metabolism*
Radioimmunoassay
Relaxin / biosynthesis*
Swine / metabolism*
Theca Cells / metabolism
Chemical
Reg. No./Substance:
9002-69-1/Relaxin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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