Document Detail


Evidence of the survival of ectopically transplanted oral mucosal epithelial stem cells after repeated wounding of cornea.
MedLine Citation:
PMID:  24769908     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Tissue engineering has become an essential tool in the development of regenerative medicine. We have developed cell sheet-based techniques for use in regenerative medicine that have already been successfully used in clinical applications. Native corneal epithelium is produced from limbal stem cells located in the transition zone between the cornea and the bulbar conjunctiva. Limbal stem cell deficiency (LSCD) is a severe defect of the limbal stem cells leading to vision loss due to conjunctival epithelial invasion and neovascularization. Rabbit LSCD models, were treated with transplantable autologous oral mucosal epithelial cell sheets fabricated on temperature-responsive cell culture surfaces, after which, the ocular surfaces were clear and smooth with no observable defects. The central part of the reconstructed ocular surface was scraped and wounded, after which proliferating epithelial cells covered the scraped area within a few days. The ocular surfaces were clear and smooth even after repeated scrapings, and consisted of only oral mucosal epithelial cells or heterogeneously mixed with corneal epithelial cells. This study demonstrates that transplanted cell sheets containing oral mucosal epithelial stem cells could reconstruct the ocular surface to maintain cornea homeostasis; moreover, they provide an ideal microenvironment to support the proliferation of remaining native limbal stem cells.Molecular Therapy (2014); doi:10.1038/mt.2014.69.
Authors:
Hiroaki Sugiyama; Masayuki Yamato; Kohji Nishida; Teruo Okano
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2014-4-28
Journal Detail:
Title:  Molecular therapy : the journal of the American Society of Gene Therapy     Volume:  -     ISSN:  1525-0024     ISO Abbreviation:  Mol. Ther.     Publication Date:  2014 Apr 
Date Detail:
Created Date:  2014-4-28     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  100890581     Medline TA:  Mol Ther     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
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