Document Detail


Evidence supporting zoonotic transmission of Cryptosporidium spp. in Wisconsin.
MedLine Citation:
PMID:  17005736     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Cryptosporidium hominis and Cryptosporidium parvum are the primary species of Cryptosporidium that infect humans. C. hominis has an anthroponotic transmission cycle, while C. parvum is zoonotic, infecting cattle and other ruminants, in addition to humans. Most cryptosporidiosis outbreaks in the United States have been caused by C. hominis, and this species is often reported as the primary cause of cryptosporidiosis in this country. However, outbreaks account for only 10% of the overall cryptosporidiosis cases, and there are few data on the species that cause sporadic cases. The present study identified the species/genotypes and subgenotypes of Cryptosporidium in 49 cases of sporadic cryptosporidiosis in Wisconsin during the period from 2003 to 2005. The species/genotype of isolates was determined by PCR restriction fragment length polymorphism analysis of the 18S rRNA and Cryptosporidium oocyst wall protein genes. The C. parvum and C. hominis isolates were subgenotyped by sequence analysis of the GP60 gene. Forty-four of 49 isolates were identified as C. parvum, and 1 was identified as C. hominis. Of the remaining isolates, one was identified as being of the cervine genotype, one was identified as being a cervine genotype variant, and two were identified as being of a novel human genotype, previously reported as W17. Nine different subgenotypes were identified within the C. parvum species, and two of these were responsible for 60% of the cases. In this study we found that most sporadic cases of cryptosporidiosis in Wisconsin are caused by zoonotic Cryptosporidium species, indicating that zoonotic transmission could be more frequently associated with sporadic cases in the United States.
Authors:
Dawn C Feltus; Catherine W Giddings; Brianna L Schneck; Timothy Monson; David Warshauer; John M McEvoy
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.     Date:  2006-09-27
Journal Detail:
Title:  Journal of clinical microbiology     Volume:  44     ISSN:  0095-1137     ISO Abbreviation:  J. Clin. Microbiol.     Publication Date:  2006 Dec 
Date Detail:
Created Date:  2006-12-07     Completed Date:  2007-02-20     Revised Date:  2013-06-07    
Medline Journal Info:
Nlm Unique ID:  7505564     Medline TA:  J Clin Microbiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  4303-8     Citation Subset:  IM    
Affiliation:
Department of Veterinary and Microbiological Sciences, North Dakota State University, 1523 Centennial Boulevard, Fargo, ND 58105, USA.
Data Bank Information
Bank Name/Acc. No.:
GENBANK/DQ640629;  DQ640630;  DQ640631;  DQ640632;  DQ640633;  DQ640634;  DQ640635;  DQ640636;  DQ640637
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MeSH Terms
Descriptor/Qualifier:
Animals
Base Sequence
Cryptosporidiosis / parasitology*,  transmission*
Cryptosporidium / classification*,  genetics,  isolation & purification*
DNA, Protozoan / chemistry,  genetics
DNA, Ribosomal / chemistry,  genetics
Genotype
Humans
Molecular Sequence Data
Phylogeny
Polymerase Chain Reaction
Polymorphism, Restriction Fragment Length
Protozoan Proteins / genetics
RNA, Ribosomal, 18S / genetics
Sequence Analysis, DNA
Sequence Homology
Wisconsin
Zoonoses
Chemical
Reg. No./Substance:
0/DNA, Protozoan; 0/DNA, Ribosomal; 0/Protozoan Proteins; 0/RNA, Ribosomal, 18S
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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