Document Detail

Evidence suggesting that the angiotensin II-sensitive intracellular Ca2+ pool is reloaded from the external space in adrenal glomerulosa cells.
MedLine Citation:
PMID:  10188615     Owner:  NLM     Status:  MEDLINE    
Adrenal glomerulosa cells prelabeled with 45Ca2+ and perifused for 10 min with 10 nM angiotensin II (AII) in a dynamic perifusion system show a biphasic response with an initial transient increase in 45Ca2+ efflux, followed by a sustained phase of increased 45Ca2+ efflux. When labeled adrenal golmerulosa cells were treated with 10 nM AII for three consecutive periods of 5 min, the transient increase in 45Ca2+ efflux was observed only in the first period. However, when 40Ca2+ was measured in the perifusate using a Ca2+-sensitive electrode coupled to the perifusion system, a transient increase in 40Ca2+ efflux was observed in each period of AII treatment. Exposing the cells to AII for 1 min, the amount of 40Ca2+ effluxed out of the cells was 58.3 +/- 8.4 nmol/10(8) cells. In contrast, when the cells were exposed to an increase in the external potassium (K+) concentration of 4 to 12 mM during 1 min of perifusion, the amount of 40Ca2+ effluxed was 16 +/- 5 nmol/10(8) cells. These results indicate that AII induces an increase in the Ca2+ concentration in a local domain outside of the plasma membrane. This Ca2+ comes from AII-induced intracellular Ca2+ depletion and may play a role in refilling intracellular Ca2+ stores.
R H Foster; A M Rojas
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Publication Detail:
Type:  In Vitro; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  General pharmacology     Volume:  32     ISSN:  0306-3623     ISO Abbreviation:  Gen. Pharmacol.     Publication Date:  1999 Feb 
Date Detail:
Created Date:  1999-05-26     Completed Date:  1999-05-26     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7602417     Medline TA:  Gen Pharmacol     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  171-7     Citation Subset:  IM    
Department of Physiology and Biophysics, Faculty of Medicine, University of Chile, Santiago.
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MeSH Terms
Aldosterone / biosynthesis
Angiotensin II / metabolism*
Calcium / metabolism*
Cells, Cultured
Dantrolene / pharmacology
Muscle Relaxants, Central / pharmacology
Potassium / metabolism
Time Factors
Zona Glomerulosa / metabolism*,  pathology
Reg. No./Substance:
0/Muscle Relaxants, Central; 11128-99-7/Angiotensin II; 52-39-1/Aldosterone; 7261-97-4/Dantrolene; 7440-09-7/Potassium; 7440-70-2/Calcium

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