Document Detail

Evidence suggesting the importance of fatty acids and the fatty acid moieties of sperm membrane phospholipids in the acrosome reaction of guinea pig spermatozoa.
MedLine Citation:
PMID:  6423766     Owner:  NLM     Status:  MEDLINE    
When guinea pig spermatozoa were preincubated 1 hr in Ca2+-free medium containing dilysocardiolipin (100--125 micrograms/ml) then exposed to Ca2+, the majority underwent an immediate acrosome reaction. Monolysocardiolipin was much less effective and the native cardiolipin was totally ineffective. Some fatty acids added to the medium could also render the spermatozoa capable of undergoing their acrosome reactions, arachidonic acid in methyl ester form being most potent. It is known that sperm membrane contains phospholipase A which cleaves membrane phospholipids into lysophospholipids and fatty acids. Most lysophospholipids are known to be potent acrosome reaction-promoting agents. As some forms of fatty acids are also potent acrosome reaction-promoting agents, both products of membrane phospholipid hydrolysis by phospholipase A (i.e., both fatty acids and lysophospholipids) may work synergistically to effect the conversion of stable sperm membranes to a fusion competent state capable of engaging in the acrosome reaction.
A D Fleming; R Yanagimachi
Related Documents :
15340236 - Comparison of the compositions of phospholipid-associated fatty acids in wild-type and ...
4085256 - Incorporation of 14c acetate into lipids of the icr mouse lens.
3754466 - The renal mitochondrial metabolism of 25-hydroxyvitamin d-3: a possible role for phosph...
15043986 - Trans fatty acids in hydrogenated fat inhibited the synthesis of the polyunsaturated fa...
19508196 - Synthesis of 4-amino-thiazole analogs of fmoc-amino acids and thiazole linked n-orthogo...
12449856 - Aspartic acid racemization and age-depth relationships for organic carbon in siberian p...
Publication Detail:
Type:  In Vitro; Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  The Journal of experimental zoology     Volume:  229     ISSN:  0022-104X     ISO Abbreviation:  J. Exp. Zool.     Publication Date:  1984 Mar 
Date Detail:
Created Date:  1984-05-24     Completed Date:  1984-05-24     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0375365     Medline TA:  J Exp Zool     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  485-9     Citation Subset:  IM    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Acrosome / drug effects*,  physiology
Arachidonic Acid
Arachidonic Acids / pharmacology
Calcium / pharmacology
Cardiolipins / pharmacology*
Fatty Acids / pharmacology*
Guinea Pigs
Membrane Lipids / physiology*
Polyvinyl Alcohol / pharmacology
Serum Albumin, Bovine / pharmacology
Spermatozoa / drug effects*
Grant Support
Reg. No./Substance:
0/Arachidonic Acids; 0/Cardiolipins; 0/Fatty Acids; 0/Membrane Lipids; 0/Serum Albumin, Bovine; 506-32-1/Arachidonic Acid; 7440-70-2/Calcium; 9002-89-5/Polyvinyl Alcohol

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Murine B cell differentiation lineages.
Next Document:  Control mechanisms governing the infectivity of Chlamydia trachomatis for HeLa cells: the role of ca...