Document Detail


Evidence suggesting the importance of fatty acids and the fatty acid moieties of sperm membrane phospholipids in the acrosome reaction of guinea pig spermatozoa.
MedLine Citation:
PMID:  6423766     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
When guinea pig spermatozoa were preincubated 1 hr in Ca2+-free medium containing dilysocardiolipin (100--125 micrograms/ml) then exposed to Ca2+, the majority underwent an immediate acrosome reaction. Monolysocardiolipin was much less effective and the native cardiolipin was totally ineffective. Some fatty acids added to the medium could also render the spermatozoa capable of undergoing their acrosome reactions, arachidonic acid in methyl ester form being most potent. It is known that sperm membrane contains phospholipase A which cleaves membrane phospholipids into lysophospholipids and fatty acids. Most lysophospholipids are known to be potent acrosome reaction-promoting agents. As some forms of fatty acids are also potent acrosome reaction-promoting agents, both products of membrane phospholipid hydrolysis by phospholipase A (i.e., both fatty acids and lysophospholipids) may work synergistically to effect the conversion of stable sperm membranes to a fusion competent state capable of engaging in the acrosome reaction.
Authors:
A D Fleming; R Yanagimachi
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Publication Detail:
Type:  In Vitro; Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  The Journal of experimental zoology     Volume:  229     ISSN:  0022-104X     ISO Abbreviation:  J. Exp. Zool.     Publication Date:  1984 Mar 
Date Detail:
Created Date:  1984-05-24     Completed Date:  1984-05-24     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0375365     Medline TA:  J Exp Zool     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  485-9     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Acrosome / drug effects*,  physiology
Animals
Arachidonic Acid
Arachidonic Acids / pharmacology
Calcium / pharmacology
Cardiolipins / pharmacology*
Fatty Acids / pharmacology*
Guinea Pigs
Male
Membrane Lipids / physiology*
Polyvinyl Alcohol / pharmacology
Serum Albumin, Bovine / pharmacology
Spermatozoa / drug effects*
Grant Support
ID/Acronym/Agency:
HD-03402/HD/NICHD NIH HHS
Chemical
Reg. No./Substance:
0/Arachidonic Acids; 0/Cardiolipins; 0/Fatty Acids; 0/Membrane Lipids; 0/Serum Albumin, Bovine; 506-32-1/Arachidonic Acid; 7440-70-2/Calcium; 9002-89-5/Polyvinyl Alcohol

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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