| Evidence for sodium-dependent active urea secretion in the deepest subsegment of the rat inner medullary collecting duct. | |
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MedLine Citation:
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PMID: 9435315 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Active reabsorption of urea appears in the initial IMCD (IMCD1) of rats fed a low-protein diet. To determine whether active urea transport also occurs in the deepest IMCD subsegment, the IMCD3, we isolated IMCDs from the base (IMCD1), middle (IMCD2), and tip (IMCD3) regions of the inner medulla from rats fed a normal protein diet and water ad libitum. IMCDs were perfused with identical perfusate and bath solutions. A significant rate of net urea secretion was present only in IMCD3s. Replacing perfusate Na+ with NMDG+ reversibly inhibited net urea secretion but replacing bath Na+ with NMDG+ or perfusate Cl- with gluconate- had no effect. Net urea secretion was significantly inhibited by: (a) 250 microM phloretin (perfusate); (b) 100 nM triamterene (perfusate); (c) 1 mM ouabain (bath); and (d) cooling the tubule to 23 degrees C. Net urea secretion was significantly stimulated by 10 nM vasopressin (bath). Next, we perfused IMCD3s from water diuretic rats (given food ad libitum) and found a significant, fivefold increase in net urea secretion. In summary, we identified a secondary active, secretory urea transport process in IMCD3s of normal rats which is upregulated in water diuretic rats. This new urea transporter may be a sodium- urea antiporter. |
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Authors:
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A Kato; J M Sands |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: The Journal of clinical investigation Volume: 101 ISSN: 0021-9738 ISO Abbreviation: J. Clin. Invest. Publication Date: 1998 Jan |
Date Detail:
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Created Date: 1998-02-05 Completed Date: 1998-02-05 Revised Date: 2009-11-18 |
Medline Journal Info:
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Nlm Unique ID: 7802877 Medline TA: J Clin Invest Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 423-8 Citation Subset: AIM; IM |
Affiliation:
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Renal Division, Department of Medicine, Emory University School of Medicine, Atlanta, Georgia 30322, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Arginine Vasopressin / pharmacology Biological Transport, Active / drug effects Kidney Medulla / secretion* Kidney Tubules, Collecting / secretion* Male Ouabain / pharmacology Phloretin / pharmacology Rats Rats, Sprague-Dawley Sodium / physiology* Urea / metabolism* |
| Grant Support | |
ID/Acronym/Agency:
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P01-DK50268/DK/NIDDK NIH HHS; R01-DK41707/DK/NIDDK NIH HHS |
| Chemical | |
Reg. No./Substance:
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113-79-1/Arginine Vasopressin; 57-13-6/Urea; 60-82-2/Phloretin; 630-60-4/Ouabain; 7440-23-5/Sodium |
| Comments/Corrections | |
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