Document Detail


Evidence for a size-sensing mechanism in animal cells.
MedLine Citation:
PMID:  15322555     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Continuously proliferating cells exactly double their mass during each cell cycle. Here we have addressed the controversial question of if and how cell size is sensed and regulated. We used erythroblasts that proliferate under the control of a constitutively active oncogene (v-ErbB) or under the control of physiological cytokines (stem cell factor, erythropoietin and v-ErbB inhibitor). The oncogene-driven cells proliferated 1.7 times faster and showed a 1.5-fold increase in cell volume. The two phenotypes could be converted into each other 24 h after altering growth factor signalling. The large cells had a higher rate of protein synthesis, together with a shortened G1 phase. Additional experiments with chicken erythroblasts and mouse fibroblasts, synchronized by centrifugal elutriation, provided further evidence that vertebrate cells can respond to cell size alterations (induced either through different growth factor signalling or DNA synthesis inhibitors) by compensatory shortening of the subsequent G1 phase. Taken together, these data suggest that an active size threshold mechanism exists in G1, which induces adjustment of cell-cycle length in the next cycle, thus ensuring maintenance of a proper balance between growth and proliferation rates in vertebrates.
Authors:
Helmut Dolznig; Florian Grebien; Thomas Sauer; Hartmut Beug; Ernst W Müllner
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2004-08-22
Journal Detail:
Title:  Nature cell biology     Volume:  6     ISSN:  1465-7392     ISO Abbreviation:  Nat. Cell Biol.     Publication Date:  2004 Sep 
Date Detail:
Created Date:  2004-09-01     Completed Date:  2004-09-28     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  100890575     Medline TA:  Nat Cell Biol     Country:  England    
Other Details:
Languages:  eng     Pagination:  899-905     Citation Subset:  IM    
Affiliation:
Institute of Molecular Pathology, The Vienna Biocenter, Institute of Medical Biochemistry, Division of Molecular Biology, Dr Bohr-Gasse 7-9, 1030 Vienna, Austria.
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Division
Cell Physiological Phenomena
Cell Size
Chickens
Erythroblasts / cytology*
G1 Phase / physiology*
Humans
Kinetics
Mice
Models, Biological
Protein Biosynthesis
S Phase / physiology
Time Factors

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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