Document Detail


Evidence for a single rat thyrotropin-beta-subunit gene: thyroidectomy increases its mRNA.
MedLine Citation:
PMID:  3839124     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We have isolated and characterized cDNAs representing the rat thyrotropin-beta-subunit. The cDNAs were prepared from poly(A)+ RNA obtained from rat pituitary glands and encode the precursor of the rat thyrotropin-beta-subunit which contains a leader or signal peptide of 20 amino acids, and an apoprotein of 118 amino acids. Blot hybridization analysis of total rat liver DNA digested with several restriction enzymes indicates the likelihood of a single gene encoding the rat thyrotropin-beta-subunit. In addition, analyses of pituitary RNA from normal and thyroidectomized rats indicate that the mRNA encoding the rat thyrotropin-beta-subunit is approximately 700 bases in length and its level increases 8--10-fold after thyroid gland ablation.
Authors:
W W Chin; J A Muccini; L Shin
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Biochemical and biophysical research communications     Volume:  128     ISSN:  0006-291X     ISO Abbreviation:  Biochem. Biophys. Res. Commun.     Publication Date:  1985 May 
Date Detail:
Created Date:  1985-07-10     Completed Date:  1985-07-10     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0372516     Medline TA:  Biochem Biophys Res Commun     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1152-8     Citation Subset:  IM    
Data Bank Information
Bank Name/Acc. No.:
GENBANK/M10902
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MeSH Terms
Descriptor/Qualifier:
Amino Acid Sequence
Animals
Base Sequence
Cloning, Molecular
DNA / genetics
Liver / metabolism
Peptide Fragments / genetics*
Pituitary Gland / metabolism
RNA, Messenger / genetics*,  metabolism
Rats
Rats, Inbred Strains
Thyroid Gland / physiology
Thyroidectomy
Thyrotropin / genetics*
Grant Support
ID/Acronym/Agency:
AM25532/AM/NIADDK NIH HHS
Chemical
Reg. No./Substance:
0/Peptide Fragments; 0/RNA, Messenger; 9002-71-5/Thyrotropin; 9007-49-2/DNA

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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