Document Detail


Evidence for sequestration of polyglutamine inclusions by Drosophila myeloid leukemia factor.
MedLine Citation:
PMID:  15936212     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Intracellular inclusions of abnormally long polyglutamine tracts and neurotoxicity are the hallmarks of several hereditary neurodegenerative disorders, including Huntington's disease (HD). In Drosophila melanogaster, dMLF, an ortholog of human myeloid leukemia factors, hMLF1 and hMLF2, suppressed polyglutamine toxicity and colocalized with the inclusions. In transfected primary rat neuronal cultures, dMLF and its orthologs reduced the morphological phenotypes and inclusions. Furthermore, dMLF reduced the recruitment of CBP and Hsp70 into the inclusions, both of which are among many essential proteins apparently trapped in the inclusions. These data suggest that a possible mechanism of suppression by dMLF is via the sequestration of polyglutamine oligomers or inclusions.
Authors:
Woo-Yang Kim; Zahra Fayazi; Xiankun Bao; Dennis Higgins; Parsa Kazemi-Esfarjani
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Molecular and cellular neurosciences     Volume:  29     ISSN:  1044-7431     ISO Abbreviation:  Mol. Cell. Neurosci.     Publication Date:  2005 Aug 
Date Detail:
Created Date:  2005-07-11     Completed Date:  2005-12-14     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  9100095     Medline TA:  Mol Cell Neurosci     Country:  United States    
Other Details:
Languages:  eng     Pagination:  536-44     Citation Subset:  IM    
Affiliation:
Department of Pharmacology and Toxicology, Center for Neuroscience, School of Medicine and Biomedical Sciences, University at Buffalo, Buffalo, NY 14214, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Cells, Cultured
Down-Regulation / genetics
Drosophila Proteins / genetics*,  metabolism*
Drosophila melanogaster
Gene Expression Regulation / physiology
HSP70 Heat-Shock Proteins / metabolism
Humans
Inclusion Bodies / genetics,  metabolism*,  pathology
Neurodegenerative Diseases / genetics,  metabolism*,  physiopathology
Neurons / metabolism*,  pathology
Nuclear Proteins / genetics,  metabolism
Peptides / metabolism*
Proteins / genetics,  metabolism
Rats
Repressor Proteins / genetics,  metabolism
Transfection
Grant Support
ID/Acronym/Agency:
NS42162/NS/NINDS NIH HHS
Chemical
Reg. No./Substance:
0/Drosophila Proteins; 0/HSP70 Heat-Shock Proteins; 0/MLF1 protein, human; 0/MLF2 protein, human; 0/Mlf protein, Drosophila; 0/Nuclear Proteins; 0/Peptides; 0/Proteins; 0/Repressor Proteins; 26700-71-0/polyglutamine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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