Document Detail

Evidence for a role of vertebrate Disp1 in long-range Shh signaling.
MedLine Citation:
PMID:  20023168     Owner:  NLM     Status:  MEDLINE    
Dispatched 1 (Disp1) encodes a twelve transmembrane domain protein that is required for long-range sonic hedgehog (Shh) signaling. Inhibition of Disp1 function, both by RNAi or dominant-negative constructs, prevents secretion and results in the accumulation of Shh in source cells. Measuring the Shh response in neuralized embryoid bodies (EBs) derived from embryonic stem (ES) cells, with or without Disp1 function, demonstrates an additional role for Disp1 in cells transporting Shh. Co-cultures with Shh-expressing cells revealed a significant reduction in the range of the contact-dependent Shh response in Disp1(-/-) neuralized EBs. These observations support a dual role for Disp1, not only in the secretion of Shh from the source cells, but also in the subsequent transport of Shh through tissue.
L Alton Etheridge; T Quinn Crawford; Shile Zhang; Henk Roelink
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Development (Cambridge, England)     Volume:  137     ISSN:  1477-9129     ISO Abbreviation:  Development     Publication Date:  2010 Jan 
Date Detail:
Created Date:  2009-12-21     Completed Date:  2010-01-27     Revised Date:  2013-05-31    
Medline Journal Info:
Nlm Unique ID:  8701744     Medline TA:  Development     Country:  England    
Other Details:
Languages:  eng     Pagination:  133-40     Citation Subset:  IM    
Department of Molecular and Cell Biology, University of California at Berkeley, 16 Barker Hall #3204, Berkeley, CA 94720-3204, USA.
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MeSH Terms
Blotting, Western
Cell Line
Cells, Cultured
Embryonic Stem Cells
Hedgehog Proteins / genetics,  metabolism*
Membrane Proteins / genetics,  physiology
RNA Interference
Signal Transduction* / genetics,  physiology
Vertebrates / physiology
Grant Support
Reg. No./Substance:
0/Hedgehog Proteins; 0/Membrane Proteins

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