Document Detail

Evidence for a role of connexin 43 in trigeminal pain using RNA interference in vivo.
MedLine Citation:
PMID:  18715894     Owner:  NLM     Status:  MEDLINE    
The importance of glial cells in the generation and maintenance of neuropathic pain is becoming widely accepted. We examined the role of glial-specific gap junctions in nociception in the rat trigeminal ganglion in nerve-injured and -uninjured states. The connexin 43 (Cx43) gap-junction subunit was found to be confined to the satellite glial cells (SGCs) that tightly envelop primary sensory neurons in the trigeminal ganglion and we therefore used Cx43 RNA interference (RNAi) to alter gap-junction function in SGCs. Using behavioral evaluation, together with immunocytochemical and Western blot monitoring, we show that Cx43 increased in the trigeminal ganglion in rats with a chronic constriction injury (CCI) of the infraorbital nerve. Reducing Cx43 expression using RNAi in CCI rats reduced painlike behavior, whereas in non-CCI rats, reducing Cx43 expression increased painlike behavior. The degree of painlike behavior in CCI rats and intact, Cx43-silenced rats was similar. Our results support previous suggestions that increases in glial gap junctions after nerve injury increases nociceptive behavior but paradoxically the reduction of gap junctions in normal ganglia also increases nociceptive behavior, possibly a reflection of the multiple functions performed by glia.
Peter T Ohara; Jean-Philippe Vit; Aditi Bhargava; Luc Jasmin
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural     Date:  2008-08-20
Journal Detail:
Title:  Journal of neurophysiology     Volume:  100     ISSN:  0022-3077     ISO Abbreviation:  J. Neurophysiol.     Publication Date:  2008 Dec 
Date Detail:
Created Date:  2008-12-08     Completed Date:  2009-04-23     Revised Date:  2013-06-05    
Medline Journal Info:
Nlm Unique ID:  0375404     Medline TA:  J Neurophysiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  3064-73     Citation Subset:  IM    
Department of Anatomy, University of California San Francisco, San Francisco, CA 95143-0452, USA.
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MeSH Terms
Analysis of Variance
Animals, Newborn
Behavior, Animal / drug effects
Connexin 43 / genetics,  metabolism*
Disease Models, Animal
Excitatory Amino Acid Transporter 1 / metabolism
Gap Junctions / drug effects,  metabolism
Gene Expression Regulation, Developmental / drug effects,  physiology
Glial Fibrillary Acidic Protein / metabolism
Neurons / drug effects,  metabolism,  pathology,  ultrastructure
Pain Measurement
RNA Interference / physiology*
RNA, Double-Stranded / pharmacology,  therapeutic use
Rats, Sprague-Dawley
Time Factors
Trigeminal Neuralgia / drug therapy,  genetics,  metabolism*,  pathology
Grant Support
Reg. No./Substance:
0/Connexin 43; 0/Excitatory Amino Acid Transporter 1; 0/Glial Fibrillary Acidic Protein; 0/RNA, Double-Stranded

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