Document Detail

Evidence for reduction of bradykinin-induced bronchoconstriction in guinea-pigs by release of nitric oxide.
MedLine Citation:
PMID:  7889267     Owner:  NLM     Status:  MEDLINE    
1. In this study the influence of nitric oxide (NO) on the bronchoconstriction induced by bradykinin in anaesthetized and artifically ventilated guinea-pigs pretreated with atropine was investigated. 2. Aerosol administration of bradykinin (0.1-1 mM, 40 breaths) caused a dose-dependent increase in lung resistance (RL): maximum increase in RL was 2.5 fold the baseline value. Pretreatment with aerosolized NG-nitro-L-arginine methyl ester (L-NAME) or NG-monomethyl-L-arginine (L-NMMA) (1 mM, 10 breaths every 5 min for 30 min), NO synthase inhibitors, markedly increased the bronchoconstrictor response to bradykinin. L-Arginine, but not D-arginine, (3 mM, 10 breaths every 5 min for 30 min) reversed the hyperresponsiveness to aerosolized bradykinin caused by L-NAME and L-NMMA. 3. L-NAME (1 mM, 10 breaths every 5 min for 30 min) increased the bronchoconstriction induced by intravenous bradykinin (1-10 nmol kg-1). L-Arginine, but not D-arginine, (10 breaths every 5 min for 30 min) reversed the hyperresponsiveness to intravenous bradykinin caused by L-NAME. 4. The increase in RL induced by capsaicin, either aerosol (10 microM, 10 breaths) or i.v. (20 nmol kg-1) was not affected by L-NAME (1 mM, 10 breaths every 5 min for 30 min). Acute resection of the vagi did not affect the bronchoconstriction evoked by bradykinin in guinea-pigs, either in the absence or presence of L-NAME (1 mM, 10 breaths every 5 min for 30 min). 4. These results suggest that, irrespective of the route of administration, bradykinin releases NO or a related molecule which exerts a bronchodilator action that opposes the bronchoconstrictor mechanisms activated by bradykinin itself.
F L Ricciardolo; J A Nadel; S Yoshihara; P Geppetti; S Yoishihara
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Publication Detail:
Type:  In Vitro; Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  British journal of pharmacology     Volume:  113     ISSN:  0007-1188     ISO Abbreviation:  Br. J. Pharmacol.     Publication Date:  1994 Dec 
Date Detail:
Created Date:  1995-04-19     Completed Date:  1995-04-19     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  7502536     Medline TA:  Br J Pharmacol     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  1147-52     Citation Subset:  IM    
Cardiovascular Research Institute, University of California, San Francisco 94143-0130.
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MeSH Terms
Arginine / analogs & derivatives,  pharmacology
Blood Pressure / drug effects
Bradykinin / administration & dosage,  pharmacology*
Bronchoconstriction / drug effects*
Capillary Permeability / drug effects
Capsaicin / pharmacology
Guinea Pigs
Hemodynamics / drug effects
Injections, Intravenous
NG-Nitroarginine Methyl Ester
Nitric Oxide / antagonists & inhibitors,  metabolism*
Pulmonary Circulation / drug effects
Vascular Resistance / drug effects
Grant Support
Reg. No./Substance:
0/Aerosols; 10102-43-9/Nitric Oxide; 17035-90-4/omega-N-Methylarginine; 404-86-4/Capsaicin; 50903-99-6/NG-Nitroarginine Methyl Ester; 58-82-2/Bradykinin; 74-79-3/Arginine
Erratum In:
Br J Pharmacol 1995 Jun;115(3):547
Note: Yoishihara, S [corrected to Yoshihara, S]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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