Document Detail

Evidence of a reduced and modified mitochondrial protein import apparatus in microsporidian mitosomes.
MedLine Citation:
PMID:  19028997     Owner:  NLM     Status:  MEDLINE    
Microsporidia are a group of highly adapted obligate intracellular parasites that are now recognized as close relatives of fungi. Their adaptation to parasitism has resulted in broad and severe reduction at (i) a genomic level by extensive gene loss, gene compaction, and gene shortening; (ii) a biochemical level with the loss of much basic metabolism; and (iii) a cellular level, resulting in lost or cryptic organelles. Consistent with this trend, the mitochondrion is severely reduced, lacking ATP synthesis and other typical functions and apparently containing only a fraction of the proteins of canonical mitochondria. We have investigated the mitochondrial protein import apparatus of this reduced organelle in the microsporidian Encephalitozoon cuniculi and find evidence of reduced and modified machinery. Notably, a putative outer membrane receptor, Tom70, is reduced in length but maintains a conserved structure chiefly consisting of tetratricopeptide repeats. When expressed in Saccharomyces cerevisiae, EcTom70 inserts with the correct topology into the outer membrane of mitochondria but is unable to complement the growth defects of Tom70-deficient yeast. We have scanned genomic data using hidden Markov models for other homologues of import machinery proteins and find evidence of severe reduction of this system.
Ross F Waller; Carole Jabbour; Nickie C Chan; Nermin Celik; Vladimir A Likic; Terrence D Mulhern; Trevor Lithgow
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-11-21
Journal Detail:
Title:  Eukaryotic cell     Volume:  8     ISSN:  1535-9786     ISO Abbreviation:  Eukaryotic Cell     Publication Date:  2009 Jan 
Date Detail:
Created Date:  2009-01-06     Completed Date:  2009-02-10     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  101130731     Medline TA:  Eukaryot Cell     Country:  United States    
Other Details:
Languages:  eng     Pagination:  19-26     Citation Subset:  IM    
School of Botany, University of Melbourne, Victoria, Australia.
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MeSH Terms
Amino Acid Sequence
Fungal Proteins / chemistry,  genetics,  metabolism*
Genetic Complementation Test
Microsporidia / chemistry,  genetics,  metabolism*
Mitochondria / chemistry,  genetics,  metabolism*
Mitochondrial Proteins / chemistry,  genetics,  metabolism*
Molecular Sequence Data
Protein Transport
Saccharomyces cerevisiae / genetics,  metabolism
Sequence Alignment
Reg. No./Substance:
0/Fungal Proteins; 0/Mitochondrial Proteins

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