| Evidence for protein-mediated fatty acid efflux by adipocytes. | |
| | |
MedLine Citation:
|
PMID: 21951599 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
|
Aim: The hormonally controlled mobilization and release of fatty acids from adipocytes into the circulation is an important physiological process required for energy homeostasis. While uptake of fatty acids by adipocytes has been suggested to be predominantly protein-mediated, it is unclear whether the efflux of fatty acids also requires membrane proteins. Methods: We used fluorescent fatty acid efflux assays and colorimetric assays for free fatty acids and glycerol to identify inhibitors with effects on fatty acid efflux, but not lipolysis, in 3T3-L1 adipocytes. We assessed the effect of these inhibitors on a fibroblast-based cell line expressing fatty acid transport protein 1, hormone-sensitive lipase, and perilipin, that presumably lacks adipocyte-speicific proteins for fatty acid efflux. Results: We identified DIDS as an inhibitor of fatty acid efflux that did not impair lipolysis or the cellular exit of glycerol, but lead to an accumulation of intracellular fatty acids. In contrast, fatty acid efflux by the reconstituted cellular model for fatty acid efflux was responsive to lipolytic stimuli, but insensitive to DIDS inhibition. Conclusion: We propose that adipocytes specifically express an as yet unidentified DIDS sensitive protein that enhances the efflux of fatty acids and therefore may lead to novel treatment approaches for obesity-related disorders characterized by abnormal lipid fluxes and ectopic triglyceride accumulation. |
| | |
Authors:
|
Amy Helene Henkin; Angelica M Ortegon; Sungyun Cho; Wen-Jun Shen; Alaric Falcon; Fredric B Kraemer; Sung-Joon Lee; Andreas Stahl |
Related Documents
:
|
1316949 - Spectroscopic properties of the nitric oxide derivative of ferrous man, horse, and rumi... 4052539 - Corrosion of a stainless steel with low nickel content under static conditions. 2127409 - Release of iron from ferritin by 6-hydroxydopamine under aerobic and anaerobic conditions. 3342069 - Perturbation of intracellular ph by dids on endocytosis of transferrin and iron uptake ... 8639489 - Mechanism of lipoxygenase inactivation by the linoleic acid analogue octadeca-9,12-diyn... 16493949 - Influence of an escherichia coli-derived phytase on nutrient utilization in broiler sta... 179909 - Inhibition by propranolol of bile acid stimulation of rabbit colonic adenylate cyclase ... 11858659 - Goadsporin, a chemical substance which promotes secondary metabolism and morphogenesis ... 11042499 - Structure of an acidic o-specific polysaccharide of the marine bacterium pseudoalteromo... |
Publication Detail:
|
Type: JOURNAL ARTICLE Date: 2011-9-26 |
Journal Detail:
|
Title: Acta physiologica (Oxford, England) Volume: - ISSN: 1748-1716 ISO Abbreviation: - Publication Date: 2011 Sep |
Date Detail:
|
Created Date: 2011-9-28 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 101262545 Medline TA: Acta Physiol (Oxf) Country: - |
Other Details:
|
Languages: ENG Pagination: - Citation Subset: - |
Copyright Information:
|
Copyright © 2011 Scandinavian Physiological Society. |
Affiliation:
|
Department of Nutritional Sciences and Toxicology, 119 Morgan Hall, University of California at Berkeley, Berkeley, CA 94720-3104, USA Korea University, Seoul, Republic of Korea Veterans Affairs Palo Alto Health Care System, 3801 Miranda Avenue, Palo Alto, CA 94304, USA Division of Endocrinology, S-025, 300 Pasteur Drive, Stanford University, Stanford, CA 94305-5103, USA. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Permanent Genetic Resources added to Molecular Ecology Resources Database 1 June 2011-31 July 2011.
Next Document: Discovering biological connections between experimental conditions based on common patterns of diffe...