Document Detail

Evidence of prokineticin dysregulation in fallopian tube from women with ectopic pregnancy.
MedLine Citation:
PMID:  20047737     Owner:  NLM     Status:  MEDLINE    
OBJECTIVE: To demonstrate expression and regulation of prokineticins (PROKs) and their receptors (PROKRs) in fallopian tube (FT) from women who are not pregnant and women with ectopic pregnancy (EP).
DESIGN: Tissue analysis.
SETTING: Large United Kingdom teaching hospital.
PATIENT(S): Women undergoing hysterectomy for benign gynecological conditions (n = 15) and surgery for EP (n = 16).
INTERVENTION(S): Quantitative reverse transcriptase-polymerase chain reaction (RT-PCR) and immunohistochemistry were used to determine FT PROK/PROKR messenger RNA (mRNA) expression and protein localization, respectively. The PROK/PROKR levels were measured in tubal explant cultures stimulated with estrogen (E) and progestogen.
MAIN OUTCOME MEASURE(S): Differential expression of PROK and PROKR.
RESULT(S): The FT PROK2 and PROKR1 mRNA levels were up-regulated during the P-dominant midluteal phase of the menstrual cycle. Increased PROKR1 expression was observed in tubal explant cultures treated with medroxy-progesterone acetate (MPA). The PROK and PROKR proteins were localized to the epithelium and smooth muscle layers of the FT. The PROKR1 and PROKR2 mRNA levels were lower in FT from women with EP compared with nonpregnant FT from the midluteal phase.
CONCLUSION(S): These data suggest a potential role for PROKs in FT function. The PROKs are known to affect smooth muscle contraction in the gut. Dysregulated PROK expression in FT could affect FT smooth muscle contractility and embryo-tubal transport, providing a potential cause for EP.
Julie L V Shaw; Fiona C Denison; Jemma Evans; Kimberley Durno; Alistair R Williams; Gary Entrican; Hilary O D Critchley; Henry N Jabbour; Andrew W Horne
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-01-04
Journal Detail:
Title:  Fertility and sterility     Volume:  94     ISSN:  1556-5653     ISO Abbreviation:  Fertil. Steril.     Publication Date:  2010 Oct 
Date Detail:
Created Date:  2010-09-27     Completed Date:  2010-11-03     Revised Date:  2013-04-29    
Medline Journal Info:
Nlm Unique ID:  0372772     Medline TA:  Fertil Steril     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1601-8.e1     Citation Subset:  IM    
Copyright Information:
Copyright © 2010 American Society for Reproductive Medicine. All rights reserved.
Centre for Reproductive Biology, Queen's Medical Research Institute, University of Edinburgh, Edinburgh, Scotland, United Kingdom.
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MeSH Terms
Cells, Cultured
Contraceptive Agents, Female / pharmacology
Epithelium / metabolism
Fallopian Tubes / cytology,  drug effects,  metabolism*
Gastrointestinal Hormones / metabolism*
Medroxyprogesterone Acetate / pharmacology
Middle Aged
Muscle Contraction / physiology
Muscle, Smooth / metabolism
Neuropeptides / metabolism*
Pregnancy, Ectopic / metabolism*
RNA, Messenger / metabolism
Receptors, G-Protein-Coupled / metabolism
Receptors, Peptide / metabolism
Vascular Endothelial Growth Factor, Endocrine-Gland-Derived / metabolism*
Young Adult
Grant Support
G0802808//Medical Research Council; G0802808(90914)//Medical Research Council; U.1276.00.004.00002.02//Medical Research Council
Reg. No./Substance:
0/Contraceptive Agents, Female; 0/Gastrointestinal Hormones; 0/Neuropeptides; 0/PROK1 protein, human; 0/PROK2 protein, human; 0/PROKR1 protein, human; 0/PROKR2 protein, human; 0/RNA, Messenger; 0/Receptors, G-Protein-Coupled; 0/Receptors, Peptide; 0/Vascular Endothelial Growth Factor, Endocrine-Gland-Derived; 71-58-9/Medroxyprogesterone Acetate

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