| Evidence for predominantly paracellular transport of thyrotropin-releasing hormone across CACO-2 cell monolayers. | |
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MedLine Citation:
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PMID: 8267614 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Uptake, transport, and metabolism of tripeptide thyrotropin-releasing hormone were examined in the human intestinal epithelial cell line, Caco-2. A linear relationship between rate and concentration was observed for both the uptake and the transport of thyrotropin-releasing hormone across Caco-2 cell monolayers. Transport of thyrotropin-releasing hormone was not affected by the presence of dipeptide glycylsarcosine, amino acid glycine, tripeptide thyrotropin-releasing hormone free acid as well as active transport inhibitors 2,4-dinitrophenol, sodium azide, ouabain, and amiloride. There was no formation of metabolites during the course of thyrotropin-releasing hormone transport across Caco-2 cells. Incubation of Caco-2 cell homogenate with thyrotropin-releasing hormone, however, showed a time-dependent hydrolysis of thyrotropin-releasing hormone and the formation of thyrotropin-releasing hormone free acid. Increased rate of transport in the presence of EDTA indicates a paracellular passive diffusion as the major route for the transport of TRH. The hydrolytic enzyme present in Caco-2 cells appeared to have little or no access to TRH during the transcellular transport across Caco-2 cell monolayers. |
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Authors:
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L S Gan; T Niederer; C Eads; D Thakker |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Biochemical and biophysical research communications Volume: 197 ISSN: 0006-291X ISO Abbreviation: Biochem. Biophys. Res. Commun. Publication Date: 1993 Dec |
Date Detail:
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Created Date: 1994-01-25 Completed Date: 1994-01-25 Revised Date: 2005-11-17 |
Medline Journal Info:
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Nlm Unique ID: 0372516 Medline TA: Biochem Biophys Res Commun Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 771-7 Citation Subset: IM |
Affiliation:
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Department of Drug Metabolism, Glaxo Inc. Research Institute Research Triangle Park, NC 27709. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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2,4-Dinitrophenol Adenocarcinoma Amiloride / pharmacology Azides / pharmacology Biological Transport / drug effects Cell Line Cell Membrane / drug effects, metabolism Colonic Neoplasms Dinitrophenols / pharmacology Dipeptides / pharmacology Edetic Acid / pharmacology Epithelium / metabolism Glycine / pharmacology Humans Intestines Kinetics Ouabain / pharmacology Sodium Azide Thyrotropin-Releasing Hormone / metabolism* Tritium Tumor Cells, Cultured |
| Chemical | |
Reg. No./Substance:
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0/Azides; 0/Dinitrophenols; 0/Dipeptides; 10028-17-8/Tritium; 24305-27-9/Thyrotropin-Releasing Hormone; 2609-46-3/Amiloride; 26628-22-8/Sodium Azide; 29816-01-1/glycylsarcosine; 51-28-5/2,4-Dinitrophenol; 56-40-6/Glycine; 60-00-4/Edetic Acid; 630-60-4/Ouabain |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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