| Evidence for a posttranscriptional effect of retinoic acid on connexin43 gene expression via the 3'-untranslated region. | |
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MedLine Citation:
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PMID: 9428648 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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All-trans retinoic acid (10(-7) M) induces cell-cell communication and the expression of the gap junction protein connexin43 in mouse F9 teratocarcinoma cells. Previous experiments revealed an increase of mRNA but no change in the transcription of connexin43, suggesting a posttranscriptional mechanism responsible for the regulation of connexin43 gene expression. In transient transfection experiments using an expression vector containing the 3'-untranslated region of the connexin43 gene downstream of the luciferase coding sequence driven by the connexin43 promoter we show here that retinoic acid enhances luciferase activity via the connexin43 3'-untranslated region due to altered stability of the mRNA. Thus, retinoic acid is able to influence connexin43 gene expression at the level of mRNA stability via elements located in the 3'-untranslated region. |
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Authors:
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A Clairmont; H Sies |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: FEBS letters Volume: 419 ISSN: 0014-5793 ISO Abbreviation: FEBS Lett. Publication Date: 1997 Dec |
Date Detail:
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Created Date: 1998-01-27 Completed Date: 1998-01-27 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0155157 Medline TA: FEBS Lett Country: NETHERLANDS |
Other Details:
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Languages: eng Pagination: 268-70 Citation Subset: IM |
Affiliation:
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Institut für Physiologische Chemie I and Biologisch-Medizinisches Forschungszentrum, Heinrich-Heine-Universität Düsseldorf, Germany. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cell Communication / drug effects, genetics Connexin 43 / genetics* Gene Expression Regulation / drug effects* Keratolytic Agents / pharmacology* Mice RNA Processing, Post-Transcriptional / drug effects Tretinoin / pharmacology* Tumor Cells, Cultured |
| Chemical | |
Reg. No./Substance:
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0/Connexin 43; 0/Keratolytic Agents; 302-79-4/Tretinoin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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