Document Detail


Evidence for a pertussis toxin-sensitive signalling pathway in the dual action of angiotensin II on growth hormone release in pituitary cell aggregates.
MedLine Citation:
PMID:  2126223     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In anterior pituitary cell aggregates cultured in the presence of the glucocorticoid dexamethasone (DEX) angiotensin II (AII) had a dual effect on growth hormone (GH) release. The peptide stimulated the release in aggregates from 2-week-old rats, whereas the peptide had an inhibitory effect in cultures from adult rats. Treatment of aggregates from adult rats with pertussis toxin (PT) reversed the inhibitory effect of AII on GH release in a stimulatory effect; PT treatment of aggregates from 18- to 20-day-old rats significantly enhanced the stimulation of GH release by AII. The effect of PT was seen only when DEX was added to the culture medium. The present data suggest that the glucocorticoid-dependent stimulus-effect coupling of AII on GH release involves both a stimulatory and an inhibitory component, the latter being abolished by PT, and that the stimulatory component predominates during immature life while the inhibitory one during adult life.
Authors:
W Robberecht; C Denef
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Cell biology international reports     Volume:  14     ISSN:  0309-1651     ISO Abbreviation:  Cell Biol. Int. Rep.     Publication Date:  1990 Nov 
Date Detail:
Created Date:  1991-03-08     Completed Date:  1991-03-08     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7708050     Medline TA:  Cell Biol Int Rep     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  1001-11     Citation Subset:  IM    
Affiliation:
Laboratory of Cell Pharmacology, School of Medicine, University of Leuven, Belgium.
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MeSH Terms
Descriptor/Qualifier:
Age Factors
Angiotensin II / pharmacology*
Animals
Cell Aggregation
Cells, Cultured
Dexamethasone / pharmacology
Female
GTP-Binding Proteins / metabolism
Growth Hormone / secretion*
Pertussis Toxin*
Pituitary Gland, Anterior / drug effects*,  growth & development,  secretion
Rats
Rats, Inbred Strains
Secretory Rate / drug effects
Signal Transduction / drug effects*
Virulence Factors, Bordetella / pharmacology*
Chemical
Reg. No./Substance:
0/Virulence Factors, Bordetella; 11128-99-7/Angiotensin II; 50-02-2/Dexamethasone; 9002-72-6/Growth Hormone; EC 2.4.2.31/Pertussis Toxin; EC 3.6.1.-/GTP-Binding Proteins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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