| Evidence for the participation of a cytosolic NADP+-dependent oxidoreductase in the catabolism of gamma-hydroxybutyrate in vivo. | |
| | |
MedLine Citation:
|
PMID: 3106576 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
The concentration of gamma-hydroxybutyrate (GHB) in brain, kidney, and muscle as well as the clearance of [1-14C]GHB in plasma have been found to be altered by the administration of a number of metabolic intermediates and drugs that inhibit the NADP+-dependent oxidoreductase, "GHB dehydrogenase," an enzyme that catalyzes the oxidation of GHB to succinic semialdehyde. Administration of valproate, salicylate, and phenylacetate, all inhibitors of GHB dehydrogenase, significantly increased the concentration of GHB in brain; salicylate increased GHB concentration in kidney, and alpha-ketoisocaproate increased GHB levels in kidney and muscle. The half-life of [1-14C]GHB in plasma was decreased by D-glucuronate, a compound that stimulates the oxidation of GHB by this enzyme and was increased by a competitive substrate of the enzyme, L-gulonate. The results of these experiments suggest a role for GHB dehydrogenase in the regulation of tissue levels of endogenous GHB. |
| | |
Authors:
|
E E Kaufman; T Nelson |
Related Documents
:
|
870646 - Effect of dietary dl-ethionine on egg laying and activities of cytoplasmic and mitochon... 2094376 - Cellular enzymatic activities in the articular cartilage of osteoarthritic and osteopor... 6626236 - The site of inhibition of porphyrin biosynthesis by an isomer of diazinon in rats. 6852056 - Perinatal development of the distributions of phosphoenolpyruvate carboxykinase and suc... 3015566 - Purification and characterization of estrogen-2/4-hydroxylase activity from rabbit hypo... 10671186 - Reductive dechlorination of tetrachloroethene to ethene by a two-component enzyme pathway. |
Publication Detail:
|
Type: Journal Article |
Journal Detail:
|
Title: Journal of neurochemistry Volume: 48 ISSN: 0022-3042 ISO Abbreviation: J. Neurochem. Publication Date: 1987 Jun |
Date Detail:
|
Created Date: 1987-06-25 Completed Date: 1987-06-25 Revised Date: 2003-11-14 |
Medline Journal Info:
|
Nlm Unique ID: 2985190R Medline TA: J Neurochem Country: UNITED STATES |
Other Details:
|
Languages: eng Pagination: 1935-41 Citation Subset: IM |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Brain / drug effects*, metabolism GABA Antagonists Glucuronates / metabolism Hydroxybutyrate Dehydrogenase / antagonists & inhibitors, metabolism* Hydroxybutyrates / metabolism* Keto Acids / pharmacology Kidney / drug effects, metabolism Male Muscles / drug effects, metabolism Phenylacetates / pharmacology Rats Rats, Inbred Strains Sodium Oxybate / metabolism* Sodium Salicylate / pharmacology Valproic Acid / pharmacology gamma-Aminobutyric Acid / analogs & derivatives |
| Chemical | |
Reg. No./Substance:
|
0/GABA Antagonists; 0/Glucuronates; 0/Hydroxybutyrates; 0/Keto Acids; 0/Phenylacetates; 502-85-2/Sodium Oxybate; 54-21-7/Sodium Salicylate; 56-12-2/gamma-Aminobutyric Acid; 692-29-5/succinic semialdehyde; 816-66-0/alpha-ketoisocaproic acid; 99-66-1/Valproic Acid; EC 1.1.1.30/Hydroxybutyrate Dehydrogenase; EC 1.1.1.61/4-hydroxybutyrate dehydrogenase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Threonine entry into rat brain after diet-induced changes in plasma amino acids.
Next Document: Factor VIII R:Ag as a prognostic parameter in intracranial haemorrhage.