Document Detail

Evidence for paracrine somatostatinergic regulation of gastrin gene expression by double-staining cytochemistry and quantitation.
MedLine Citation:
PMID:  7903328     Owner:  NLM     Status:  MEDLINE    
Gastrin is produced by gastric endocrine cells and regulates acid secretion and mucosal cell proliferation. Somatostatin inhibits gastrin secretion, and exogenous somatostatin was recently reported to decrease gastrin gene transcription and to reduce gastrin mRNA stability. Somatostatin cells extend cytoplasmic processes that contact gastrin cells and other cells. Using quantitative in situ hybridization combined with immunocytochemistry, we demonstrate that gastrin cells in close contact (< or = 0.2 micron) with somatostatin cells express significantly lower levels of gastrin mRNA than other gastrin cells. Our data show that cells with high levels of gastrin mRNA and no detectable contacts predominate at the base of the glands. Hence, the results provide evidence that endogenous somatostatin regulates gastrin gene expression in a subpopulation of gastrin cells and point to regulatory heterogeneity of these cells. This represents the first direct microscopic evidence for paracrine gene regulation. The new quantitative double-staining approach may be generally useful for studies of cell interactions and cell sociology.
L I Larsson; D M Houggaard
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society     Volume:  42     ISSN:  0022-1554     ISO Abbreviation:  J. Histochem. Cytochem.     Publication Date:  1994 Jan 
Date Detail:
Created Date:  1994-01-26     Completed Date:  1994-01-26     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9815334     Medline TA:  J Histochem Cytochem     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  37-40     Citation Subset:  IM    
Department of Molecular Cell Biology, Statens Seruminstitut, Copenhagen, Denmark.
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MeSH Terms
DNA / analysis,  genetics
Gastrins / analysis,  genetics*,  secretion
Gene Expression Regulation / genetics*
Image Processing, Computer-Assisted
Immunohistochemistry / methods
In Situ Hybridization
RNA, Messenger / analysis,  genetics
Rats, Wistar
Somatostatin / analysis,  physiology*
Stomach / chemistry,  physiology*
Transcription, Genetic
Reg. No./Substance:
0/Gastrins; 0/RNA, Messenger; 51110-01-1/Somatostatin; 9007-49-2/DNA

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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