| Evidence for nerve growth factor-potentiating activities of the nonpeptidic compound SR 57746A in PC12 cells. | |
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MedLine Citation:
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PMID: 7722483 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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SR 57746A (1-[2-(naphth-2-yl)ethyl]-4-(3-trifluoromethylphenyl)-1,2,5,6- tetrahydropyridine hydrochloride) exhibits neurotrophic activities in vivo and in vitro. We used the rat pheochromocytoma PC12 cell line to investigate in vitro cellular changes induced by SR 57746A. A significant increase in the percentage of cells bearing neurite-like processes was obtained in cells treated by SR 57746A and nerve growth factor (NGF) compared with NGF treatment alone. SR 57746A added alone, however, had no effect on morphogenesis or on survival of cells in serum-free medium. In contrast, SR 57746A induced a "priming" effect on PC12 cells for neurite outgrowth within 6 h of addition of the protein tyrosine kinase inhibitor genistein. An increase in alpha-actinin content resulted from treatment with SR 57746A. Expression of NGF-mediated acetylcholinesterase and choline acetyltransferase was enhanced within 5 days by SR 57746A. The molecule also induced rapid F-actin redistribution. Within 2 min of incubation, outgrowth of F-actin-containing filopodia was clearly visible at the cell periphery, as previously shown with NGF. It is interesting that this effect of SR 57746A could be mimicked by protein tyrosine kinase inhibitors and abolished by preincubation with sodium orthovanadate, a protein tyrosine phosphatase inhibitor. |
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Authors:
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A Pradines; M Magazin; P Schiltz; G Le Fur; D Caput; P Ferrara |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Journal of neurochemistry Volume: 64 ISSN: 0022-3042 ISO Abbreviation: J. Neurochem. Publication Date: 1995 May |
Date Detail:
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Created Date: 1995-05-23 Completed Date: 1995-05-23 Revised Date: 2005-07-21 |
Medline Journal Info:
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Nlm Unique ID: 2985190R Medline TA: J Neurochem Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 1954-64 Citation Subset: IM |
Affiliation:
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Sanofi Recherche, Labège, France. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Acetylcholinesterase
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metabolism Actinin / metabolism Actins / analysis Animals Cell Differentiation / drug effects Cell Survival / drug effects Culture Media Drug Synergism Genistein Isoflavones / pharmacology Naphthalenes / pharmacology* Nerve Growth Factors / pharmacology* Neurites / drug effects, ultrastructure PC12 Cells / metabolism, ultrastructure* Pyridines / pharmacology* Rats Serotonin Antagonists / pharmacology* Signal Transduction / drug effects Tumor Cells, Cultured |
| Chemical | |
Reg. No./Substance:
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0/Actins; 0/Culture Media; 0/Isoflavones; 0/Naphthalenes; 0/Nerve Growth Factors; 0/Pyridines; 0/Serotonin Antagonists; 11003-00-2/Actinin; 135354-02-8/xaliproden; 446-72-0/Genistein; EC 3.1.1.7/Acetylcholinesterase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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