Document Detail


Evidence for nerve growth factor-potentiating activities of the nonpeptidic compound SR 57746A in PC12 cells.
MedLine Citation:
PMID:  7722483     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
SR 57746A (1-[2-(naphth-2-yl)ethyl]-4-(3-trifluoromethylphenyl)-1,2,5,6- tetrahydropyridine hydrochloride) exhibits neurotrophic activities in vivo and in vitro. We used the rat pheochromocytoma PC12 cell line to investigate in vitro cellular changes induced by SR 57746A. A significant increase in the percentage of cells bearing neurite-like processes was obtained in cells treated by SR 57746A and nerve growth factor (NGF) compared with NGF treatment alone. SR 57746A added alone, however, had no effect on morphogenesis or on survival of cells in serum-free medium. In contrast, SR 57746A induced a "priming" effect on PC12 cells for neurite outgrowth within 6 h of addition of the protein tyrosine kinase inhibitor genistein. An increase in alpha-actinin content resulted from treatment with SR 57746A. Expression of NGF-mediated acetylcholinesterase and choline acetyltransferase was enhanced within 5 days by SR 57746A. The molecule also induced rapid F-actin redistribution. Within 2 min of incubation, outgrowth of F-actin-containing filopodia was clearly visible at the cell periphery, as previously shown with NGF. It is interesting that this effect of SR 57746A could be mimicked by protein tyrosine kinase inhibitors and abolished by preincubation with sodium orthovanadate, a protein tyrosine phosphatase inhibitor.
Authors:
A Pradines; M Magazin; P Schiltz; G Le Fur; D Caput; P Ferrara
Related Documents :
18926803 - Pregnenolone protects the pc-12 cell line against amyloid beta peptide toxicity but its...
1986033 - Age-associated and cell-type specific changes in ngf requirement for neurite regenerati...
1268043 - A quantitative assessment of the structural changes the rat's liver following obstructi...
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of neurochemistry     Volume:  64     ISSN:  0022-3042     ISO Abbreviation:  J. Neurochem.     Publication Date:  1995 May 
Date Detail:
Created Date:  1995-05-23     Completed Date:  1995-05-23     Revised Date:  2005-07-21    
Medline Journal Info:
Nlm Unique ID:  2985190R     Medline TA:  J Neurochem     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1954-64     Citation Subset:  IM    
Affiliation:
Sanofi Recherche, Labège, France.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Acetylcholinesterase / metabolism
Actinin / metabolism
Actins / analysis
Animals
Cell Differentiation / drug effects
Cell Survival / drug effects
Culture Media
Drug Synergism
Genistein
Isoflavones / pharmacology
Naphthalenes / pharmacology*
Nerve Growth Factors / pharmacology*
Neurites / drug effects,  ultrastructure
PC12 Cells / metabolism,  ultrastructure*
Pyridines / pharmacology*
Rats
Serotonin Antagonists / pharmacology*
Signal Transduction / drug effects
Tumor Cells, Cultured
Chemical
Reg. No./Substance:
0/Actins; 0/Culture Media; 0/Isoflavones; 0/Naphthalenes; 0/Nerve Growth Factors; 0/Pyridines; 0/Serotonin Antagonists; 11003-00-2/Actinin; 135354-02-8/xaliproden; 446-72-0/Genistein; EC 3.1.1.7/Acetylcholinesterase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Synergistic stimulation of interleukin 6 release and gene expression by phorbol esters and interleuk...
Next Document:  Effect of peroxynitrite on the mitochondrial respiratory chain: differential susceptibility of neuro...