Document Detail


Evidence of native-like substructure(s) in polypeptide chains of carbonic anhydrase deposited into insoluble aggregates during thermal unfolding.
MedLine Citation:
PMID:  17882537     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A non-specific protease, subtilisin, was used to probe the existence of folded structure in thermal aggregates of bovine carbonic anhydrase-II (BCA). BCA aggregates and native BCA were subjected to proteolysis and electrophoretic analyses which revealed the accumulation of polypeptide fragments of similar size, indicating survival of similar sections of folded structure burying peptide bonds away from scission in the two samples. N-terminal sequencing revealed that the termini of size-matched fragments from the two samples were either identical, or located very close to each other, and predominantly on the surface of the 3-dimensional structure of native BCA. The susceptibility to proteolysis of very nearly the same sites in the two samples suggests that native-like elements of structure survive within BCA aggregates. The finding that thermal aggregation can involve interactions among molecules retaining elements of native-like structure, suggests that complete chain unfolding may not be a necessary prerequisite for all aggregation.
Authors:
Swati Sharma; Purnananda Guptasarma
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The protein journal     Volume:  27     ISSN:  1572-3887     ISO Abbreviation:  Protein J.     Publication Date:  2008 Jan 
Date Detail:
Created Date:  2008-02-05     Completed Date:  2008-04-15     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101212092     Medline TA:  Protein J     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  50-8     Citation Subset:  IM    
Affiliation:
Institute of Microbial Technology, Chandigarh, 160 036, India.
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MeSH Terms
Descriptor/Qualifier:
Amino Acid Sequence
Animals
Carbonic Anhydrases / chemistry*
Cattle
Molecular Sequence Data
Protein Folding*
Chemical
Reg. No./Substance:
EC 4.2.1.1/Carbonic Anhydrases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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