| Evidence for microRNA involvement in exercise-associated neutrophil gene expression changes. | |
| | |
MedLine Citation:
|
PMID: 20110541 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Exercise leads to a rapid change in the profile of gene expression in circulating neutrophils. MicroRNAs (miRNAs) have been discovered to play important roles in immune function and often act to attenuate or silence gene translation. We hypothesized that miRNA expression in circulating neutrophils would be affected by brief exercise. Eleven healthy men (19-30 yr old) performed 10, 2-min bouts of cycle ergometer exercise interspersed with 1-min rest at a constant work equivalent to approximately 76% of maximal oxygen uptake (Vo(2 max)). We used the Agilent Human miRNA V2 Microarray. A conservative statistical approach was used to determine that exercise significantly altered 38 miRNAs (20 had lower expression). Using RT-PCR, we verified the expression level changes from before to after exercise of seven miRNAs. In silico analysis showed that collectively 36 miRNAs potentially targeted 4,724 genes (2 of the miRNAs had no apparent gene targets). Moreover, when we compared the gene expression changes (n = 458) in neutrophils that have been altered by exercise, as previously reported, with the miRNAs altered by exercise, we identified three pathways, Ubiquitin-mediated proteolysis, Jak-STAT signaling pathway, and Hedgehog signaling pathway, in which an interaction of miRNA and gene expression was plausible. Each of these pathways is known to play a role in key mechanisms of inflammation. Brief exercise alters miRNA profile in circulating neutrophils in humans. These data support the hypothesis that exercise-associated changes in neutrophil miRNA expression play a role in neutrophil gene expression in response to physical activity. |
| | |
Authors:
|
Shlomit Radom-Aizik; Frank Zaldivar; Stacy Oliver; Pietro Galassetti; Dan M Cooper |
Publication Detail:
|
Type: Journal Article; Research Support, N.I.H., Extramural Date: 2010-01-28 |
Journal Detail:
|
Title: Journal of applied physiology (Bethesda, Md. : 1985) Volume: 109 ISSN: 1522-1601 ISO Abbreviation: J. Appl. Physiol. Publication Date: 2010 Jul |
Date Detail:
|
Created Date: 2010-06-30 Completed Date: 2010-10-14 Revised Date: 2011-08-01 |
Medline Journal Info:
|
Nlm Unique ID: 8502536 Medline TA: J Appl Physiol Country: United States |
Other Details:
|
Languages: eng Pagination: 252-61 Citation Subset: IM |
Affiliation:
|
Pediatric Exercise Research Center, Department of Pediatrics, University Children's Hospital, University of California-Irvine, Orange, CA 92868, USA. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Adult Exercise* Gene Expression Profiling Gene Expression Regulation* Hedgehog Proteins / metabolism Humans Janus Kinases / metabolism Male MicroRNAs / metabolism* Neutrophils / metabolism* STAT Transcription Factors / metabolism Ubiquitin / metabolism Ubiquitination Young Adult |
| Grant Support | |
ID/Acronym/Agency:
|
MO1-RR00827/RR/NCRR NIH HHS; P01-HD-048721/HD/NICHD NIH HHS; R01-HL-080947/HL/NHLBI NIH HHS |
| Chemical | |
Reg. No./Substance:
|
0/Hedgehog Proteins; 0/MicroRNAs; 0/STAT Transcription Factors; 0/Ubiquitin; EC 2.7.10.2/Janus Kinases |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Glia modulation of the extracellular milieu as a factor in central CO2 chemosensitivity and respirat...
Next Document: Near-infrared spectroscopy and indocyanine green derived blood flow index for non-invasive measureme...