Document Detail

Evidence for many resolvable structures within conformation types of electrosprayed ubiquitin ions.
MedLine Citation:
PMID:  16571016     Owner:  NLM     Status:  MEDLINE    
A new two-dimensional ion mobility spectrometry approach combined with mass spectrometry has been used to examine ubiquitin ions in the gas phase. In this approach ions are separated in an initial drift tube into conformation types (defined by their collision cross sections) and then a gate is used to introduce a narrow distribution of mobility-separated ions into a second drift tube for subsequent separation. The results show that upon selection a narrow peak shape is retained through the second drift tube. This requires that at 300 K the selected distribution does not interconvert substantially within the broader range of structures associated with the conformation type within the approximately 10-20 ms time scale of these experiments. For the [M + 7H]7+ ion, it appears that many ( approximately 5-10) narrow selections can be made across each of the compact, partially-folded, and elongated conformer types, defined previously (Int. J. Mass Spectrom. 1999, 187, 37-47).
Stormy L Koeniger; Samuel I Merenbloom; David E Clemmer
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  The journal of physical chemistry. B     Volume:  110     ISSN:  1520-6106     ISO Abbreviation:  J Phys Chem B     Publication Date:  2006 Apr 
Date Detail:
Created Date:  2006-03-30     Completed Date:  2007-07-31     Revised Date:  2007-12-03    
Medline Journal Info:
Nlm Unique ID:  101157530     Medline TA:  J Phys Chem B     Country:  United States    
Other Details:
Languages:  eng     Pagination:  7017-21     Citation Subset:  IM    
Department of Chemistry, Indiana University, Bloomington, Indiana 47405, USA.
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MeSH Terms
Ions / chemistry*
Spectrometry, Mass, Electrospray Ionization
Tandem Mass Spectrometry
Ubiquitin / chemistry*
Grant Support
AG-024547-01/AG/NIA NIH HHS; P41-RR018942/RR/NCRR NIH HHS
Reg. No./Substance:
0/Ions; 0/Ubiquitin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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