Document Detail

Evidence for inactivation of DNA repair in frozen and thawed mammalian cells.
MedLine Citation:
PMID:  335236     Owner:  NLM     Status:  MEDLINE    
A variety of cell strains and lines were frozen and thawed by conventional techniques for cell storage. Following thawing, extracts of cells were prepared and incubated with UV-irradiated E. coli DNA. Thymine dimer excision activity present in extracts of unfrozen cells was lost in extracts of recently thawed cells. The ability to exercise dimers was restored after about 40 h post-thawing, but the recovery was inhibited if cells were cultured in the presence of puromycin. Correlating with the loss of dimer excising activity there was a reduced cell viability as measured by trypan blue dye exclusion.
H Slor; T Lev-Sobe; E C Friedberg
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Mutation research     Volume:  45     ISSN:  0027-5107     ISO Abbreviation:  Mutat. Res.     Publication Date:  1977 Oct 
Date Detail:
Created Date:  1977-12-29     Completed Date:  1977-12-29     Revised Date:  2009-10-27    
Medline Journal Info:
Nlm Unique ID:  0400763     Medline TA:  Mutat Res     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  137-45     Citation Subset:  IM    
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MeSH Terms
Cell Line
Cell Survival
DNA Repair*
DNA, Bacterial / metabolism
Escherichia coli
Hela Cells / metabolism
Puromycin / pharmacology
Pyrimidine Dimers / metabolism
Reg. No./Substance:
0/DNA, Bacterial; 0/Pyrimidine Dimers; 53-79-2/Puromycin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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