Document Detail

Evidence in support of a chemiosmotic mechanism for stimulated prolactin release.
MedLine Citation:
PMID:  2101544     Owner:  NLM     Status:  MEDLINE    
In order to assess the involvement of a chemiosmotic mechanism in exocytotic prolactin (PRL) release, dispersed anterior pituitary cells from female rats were used. After primary culture they were incubated in media containing bicarbonate or isethionate ions, probenecid and carbonylcyanide p-trifluoromethoxyphenyl-hydrazone (CCTP). Cells incubated without these additives or stimulated with dibutyryladenosine 3':5'-cyclic monophosphate served as controls. Further, a crude secretory granule suspension was used to study the effects of elevated osmotic pressures. The inhibition of anion transport with probenecid always significantly reduced PRL secretion, whereas bicarbonate ions had the opposite effect. The proton ionophore CCTP only inhibited stimulated release, and the substitution of chloride ions by isethionate was ineffective. Finally, it could be observed that elevated osmotic pressures reduced the lysis of isolated secretory granules when bicarbonate ions were present in the incubation medium. The results indicate that a mechanism requiring protons and bicarbonate ions is involved in stimulated PRL release and that a different mechanism, independent from proton gradients, should be considered for basal PRL secretion.
M Bilinski
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Acta physiologica et pharmacologica latinoamericana : organo de la Asociación Latinoamericana de Ciencias Fisiológicas y de la Asociación Latinoamericana de Farmacología     Volume:  40     ISSN:  0326-6656     ISO Abbreviation:  Acta Physiol Pharmacol Latinoam     Publication Date:  1990  
Date Detail:
Created Date:  1991-09-09     Completed Date:  1991-09-09     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8409686     Medline TA:  Acta Physiol Pharmacol Latinoam     Country:  ARGENTINA    
Other Details:
Languages:  eng     Pagination:  381-9     Citation Subset:  IM    
Instituto de Neurobiología, Buenos Aires, Argentina.
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MeSH Terms
Bicarbonates / pharmacology
Exocytosis / drug effects*
Hydrogen-Ion Concentration
Mice, Inbred Strains
Osmotic Pressure / drug effects
Probenecid / pharmacology*
Prolactin / secretion*
Reg. No./Substance:
0/Bicarbonates; 57-66-9/Probenecid; 9002-62-4/Prolactin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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