Document Detail

Evidence for the importance of cysteine and arginine residues in Pseudomonas fluorescens UK-1 pantoate dehydrogenase.
MedLine Citation:
PMID:  6773579     Owner:  NLM     Status:  MEDLINE    
Homogeneous pantoate dehydrogenase (D-pantoate:NAD+ 4-oxidoreductase, EC was shown to be sensitive to inactivation by p-chloromercuribenzoate (100 microM), 5,5'-dithiobis(2-nitrobenzoic acid) (1 mM), iodoacetic acid (1 mM) and phenylglyoxal (5.3 mM). Potassium D-pantoate and NAD protected against inactivation by p-chloromercuribenzoate, 5,5'-dithiobis (2-nitrobenzoic acid) and iodoacetic acid. NAD and D-pantoate also provided substantial protection against inactivatrion by phenylglyoxal. Titration of the sulphydryl groups by 5,5'-dithiobis(2-nitrobenzoic acid) and incorporation of [14C]carboxymethyl revealed that there are two cysteine residues which are modified and one of those is essential for activity. In the presence of NAD and D-pantoate, incorporation of [14C]phenylglyoxal was decreased by 0.42 mol/mol of subunit.
T Myöhänen; P Mäntsälä
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Biochimica et biophysica acta     Volume:  614     ISSN:  0006-3002     ISO Abbreviation:  Biochim. Biophys. Acta     Publication Date:  1980 Aug 
Date Detail:
Created Date:  1980-11-24     Completed Date:  1980-11-24     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0217513     Medline TA:  Biochim Biophys Acta     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  266-73     Citation Subset:  IM    
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MeSH Terms
Alcohol Oxidoreductases / antagonists & inhibitors*
Arginine / metabolism*
Chloromercuribenzoates / pharmacology
Cysteine / metabolism*
Dithionitrobenzoic Acid / pharmacology
Hydroxybutyrates / antagonists & inhibitors,  pharmacology
Iodoacetates / pharmacology
NAD / pharmacology
Phenylglyoxal / pharmacology
Pseudomonas fluorescens / enzymology*
Reg. No./Substance:
0/Chloromercuribenzoates; 0/Hydroxybutyrates; 0/Iodoacetates; 1074-12-0/Phenylglyoxal; 470-29-1/pantoic acid; 52-90-4/Cysteine; 53-84-9/NAD; 69-78-3/Dithionitrobenzoic Acid; 74-79-3/Arginine; EC 1.1.-/Alcohol Oxidoreductases; EC dehydrogenase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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